血清脂蛋白胆固醇水平与HDL亚类组成的关系.pdf

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1、四川大学学报（医学版）2005；36（1）：2427JSichuanUniv（MedSciEdi）*血清脂蛋白胆固醇水平与HDL亚类组成的关系11A2111勾蓝图，傅明德，徐燕华，颜丙玉，杨雨叶，刘宇1.四川大学华西基础医学与法医学院生物化学与分子生物学教研室（成都610041）；2.华神集团博士后工作站【摘要】目的探讨血清HDL-C、LDL-C水平对HDL亚类组成及含量的影响。方法采用双向电泳-免疫印迹检测法分析了292例受试者血清HDL亚类的组成及含量。结果随血清HDL-C水平逐渐降低，pre!1-HDL、HDL3b含量呈增加趋势，与高HDL-C组比较，低HDL-C组pre!1

2、-HDL（P<0.01）、HDL3b（P<0.05）含量显著增加；相反，HDL2b、HDL2a含量呈减少趋势，与高HDL-C组比较，中HDL-C组及低HDL-C组HDL2b、HDL2a含量显著减少（P<0.01）。随血清LDL-C水平逐渐升高，pre!1-HDL、HDL3C、HDL3b含量呈增加趋势，与正常LDL-C组比较，高LDL-C组（P<0.05）和极高LDL-C组（P<0.01）pre!1-HDL、HDL3b含量显著增加，极高LDL-C组HDL3C含量也显著增加（P<0.01）；相反，HDL2b含量呈减少趋势，与正常LDL-C组比较，高LDL-C组（P<0.05）和极高LD

3、L-C组（P<0.01）HDL2b含量显著减少。结论随血清HDL-C水平降低或LDL-C水平升高，均有小颗粒HDL含量增加及大颗粒HDL含量减少趋势，且血清HDL-C水平与大颗粒HDL2a、HDL2b含量的变化关系更为明显。【关键词】HDL亚类HDL-CLDL-C双向电泳-免疫印迹检测法【中图分类号】R344.6AAssociationBetweenSerumLipoprotein-cholesterolLevelsandHDLSubpopulationsGOULan-tu，FUMing-de，XUYan-hua，YANBing-yu，YANGYu-ye，LIUYu.Biochem

4、istryandMolecularBiologyLaboratory，WestChinaSchoolofPreclinicalandForensicMedicine，SichuanUniuersity，Chengdu610041，China【Abstract】ObjectiveToinvestigatetheinfIuenceofserumHDL-CorLDL-CIeveIsonthecomponentsofserumHDLsubpopuIations.MethodsApoIipoprotein（apo）A-"contentsofserumHDLsubpopuIationsin29

5、2subjectsweredeterminedbytwo-dimensionaIgeIeIectrophoresisinconjunctionwithimmunodection.ResultsWiththedecreaseofser-umHDL-CIeveIs，theapoA-"contentsofpre!1-HDLandHDL3bincreasedandweresignificantIyhigher（P<0.01；P<0.05）intheIowHDL-CgroupthaninthehighHDL-Cgroup.Butonthecontrary，withthedecreaseofs

6、erumHDL-CIev-eIs，theapoA-"contentsofHDL2bandHDL2adecreasedandweresignificantIyIower（P<0.01）inthemiddIeandIowHDL-CgroupsthaninthehighHDL-Cgroup.WiththeincreaseofserumLDL-CIeveIs，theapoA-"contentsofpre!1-HDL，HDL3CandHDL3bincreased；theapoA-Icontentsofpre!1-HDLandHDL3bweresignificantIyhigherintheh

7、ighLDL-Cgroup（P<0.05）andveryhighLDL-Cgroup（P<0.01），andthoseofHDL3CwereaIsosignificantIyhigherintheveryhighLDL-Cgroup（P<0.01）.Butonthecontrary，withtheincreaseofLDL-CIeveIs，theapoA-"contentsofHDL2bdecreasedandweresignificantIyIowerinthehi