甘薯套作对根际土壤细菌群落多样性及植株氮素吸收的影响-论文.pdf

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1、作物学报ACTAAGRONOMICASINICA2012，38(2)：333—343http：／／www．chinacrops．org／zwxb／ISSN0496—3490；CODENTSHPA9E—mail：xbzw@chinajournaLnet．cnDoI．10．3724／SP．J．1006．2012．00333小麦／T-．米／大豆和小麦／t米／甘薯套作对根际土壤细菌群落多样性及植株氮素吸收的影响雍太文杨文钰向达兵朱贞颖四川农业大学农学院，四川温江611130摘要：为探讨小麦／玉米／大豆和小麦／玉米／甘薯套作体系中根际细菌群落多样性与作物氮素高效吸收的差异特性及二者间

2、的关系，应用变性梯度凝胶电泳(DGGE)技术研究了小麦一大豆(A1)、小麦一甘薯(A2)、玉米(A3)、小麦／t米／大豆(A4)和小麦／t米／甘薯(A5)5种种植模式的根际细菌群落多样性。结果表明，与A1、A2、A3及A5相比，A4套作提高了各作物在开花期(或吐丝期)与成熟期的籽粒吸氮量、地上部总吸氮量和Shannon—Weinerindex多样性指数(H)。处理间的吸氮量与日的变化规律为套作>单作、大豆茬口>甘薯茬口，以A4处理最高。不同种植模式下DGGE图谱条带的数量及亮度有较大区别，且有几条特征性条带发生了明显变化。不同种植模式间的细菌群落结构相似性较低，群落相似度系

3、数()表现为套作与套作间>套作与单作间；A4与A5间的相对较小，二者间的细菌群落结构差异较大。A4模式有利于提高根际细菌群落多样性，增强植株对氮素的吸收能力。关键词：小麦／t米／大豆套作；氮素吸收；细菌群落多样性；PCR—DGGEEffectofWheat／Maize／SoybeanandWheat／Maize／SweetPotatoRelayStripIntercroppingonBacterialCommunityDiversityofRhizosphereSoilandNi-trogenUptakeofCropsYONGTai—Wen，YANGWen—Yu，XIANG

4、Da．Bing，andZHUZhen—YingAgronomyCollege，SichuanAgriculturalUniversity,Wenjiang611130，ChmaAbstract：Theaimofthisstudywastounderstandtherelationshipbetweendiversityofrhizospherebacterialcomr0unityandnitrogenuptakeofcropsintworelaystripintercroppingsystems：wheat／maize／soybearlandwheat／maize／swe

5、etpotato．Weana-lyzedthediversitiesofrhizospherebacterialcommunityinfivecroppingsystemsusingdenaturinggradientgelelectrophoresis(DGGE)basedon16SrDNA．Thecroppingsystemsincludedwheat-soybean(A1)，wheat-sweetpotato(A2)，maizesinglecrop—ping(A3)，wheat／maize／soybearl(A4)，andwheat／maize／sweetpotato

6、(A5)．Comparedtothesolecroppingsystems(A1，A2，andA3treatments)，theA4treatmentshowedincreasesingrainnitrogenuptakeandtotalnitrogenuptakeamountsofabovegroundofcropsatbothflowering(orsilking)andmatnritystages，andtheShannon—Weinerindicesforrhizospherebacterialcommunitydiversitywasalsoincreasedsi

7、gnificantly．ThevaluesofnitrogenuptakeandtheShannon—Weinerindexintherelaystripinter-croppingsystemsweregreaterthanthoseinsolecroppingsystems．andthevaluesinthetreatmentwjtl1soybearlstubbleweregreaterthanthoseinthetreatmentwithsweetpotatostubble．Thenumberandstren