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ID:37218742
大小:1.30 MB
页数:12页
时间:2019-05-19
《低磷胁迫对番茄根系生长及根际土壤细菌多样性的影响》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、园艺学报,2016,43(3):473–484.ActaHorticulturaeSinicadoi:10.16420/j.issn.0513-353x.2015-0919;http://www.ahs.ac.cn473低磷胁迫对番茄根系生长及根际土壤细菌多样性的影响1111,2,*李荣坦,姚华开,刘岳飞,杨尚东12(广西大学农学院园艺系,南宁530004;广西农业科学院,广西甘蔗遗传改良重点实验室,南宁530007)摘要:以低磷胁迫和平衡施肥处理的番茄根系及根际土壤为研究对象,采用WinRHIZO根系分析
2、系统、稀释平板法、PCR-DGGE等分析技术,研究低磷胁迫对番茄根系生长、根际土壤生物学特性以及细菌多样性的影响。结果表明:低磷胁迫导致番茄根系总根长、总根表面积、总根体积和根尖数减少;导致根际土壤中可培养微生物(细菌、真菌和放线菌)数量、酶活性、微生物生物量(C、N、P)以及细菌的多样性指数(H)、丰富度(S)和均匀度指数(Eh)等表征土壤肥力及健康状态的指标下降;导致部分诸如甲基杆菌属(Methylobacteriumsp.)等具有溶磷功能的菌群缺失;平衡施肥对番茄的正常生长和提升土壤肥力以及维护土壤健
3、康具有极其重要的作用。关键词:番茄;低磷胁迫;根系;土壤细菌多样性;PCR-DGGE中图分类号:S641.2文献标志码:A文章编号:0513-353X(2016)03-0473-12EffectofLowPhosphorusStressonRootGrowthandSoilBacterialDiversityinRhizosphereofTomatoes1111,2,*LIRong-tan,YAOHua-kai,LIUYue-fei,andYANGShang-dong12(DepartmentofHorti
4、culture,GuangxiUniversity,Nanning530004,China;GuangxiKeyLaboratoryofSugarcaneGeneticImprovement,GuangxiAcademyofAgriculturalSciences,Nanning530007,China)Abstract:Basedonthetraditionalandmodernanalyzedtechniques,suchasWinRHIZOrootanalysissystem,dilution-pla
5、temethod,PCR-DGGEetc.,theeffectoflowphosphorusstressonrootgrowth,soilmicrobialpropertiesandbacterialdiversityunderlowphosphorusstresswereanalyzed.Theresultsshowedthattherootlength,surfacearea,volumeandtipnumberwerealldecreasedunderlowphosphorusstress.Addit
6、ionally,soilmicrobialbiomass,enzymeactivities,microbialbiomass(C,N,P)andsoilbacterialdiversityindex(H),richness(S)andevennessindex(Eh)wereallalsodecreasedunderlowphosphorusstress.However,somebacteriagroups,suchasMethylbacterium(sp.),whichhavethefunctionofs
7、olublephosphorus,disappearinrhizospheresoilunderlowphosphorusstresscondition.Moreover,itindicatedthatthedegradationofsoilfertilityandsoilbacterialcommunitystructureinrhizospherewerethemainreasonforthepoorgrowthoftomatoes.Thebalancefertilizationhasanimporta
8、ntfunctioninimprovingthegrowthoftomatoes,soilfertilityandhealth.收稿日期:2015–12–30;修回日期:2016–03–04基金项目:国家自然科学基金项目(31360506);南宁市科学研究与技术开发计划项目(20132313);广西农业科学院广西甘蔗遗传改良重点实验室开放课题(12-K-05-02)*通信作者Authorforcorrespond
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