大豆疫病拮抗细菌的筛选及生防作用-论文.pdf

《大豆疫病拮抗细菌的筛选及生防作用-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、安徽农业大学学报，2012，39(5)：677．681JournalofAnhuiAgriculturalUniversity网络出版时间：2012．8．2712：04：03[URL】http：／／www．cnki．net／kcms／detail／34．1162．S．20120827．1204．028．html大豆疫病拮抗细菌的筛选及生防作用杨光红，高智谋，曹舜，江涛，汪涛(1．安徽农业大学植物保护学院，合肥230036；2安徽农业科学研究院植物保护研究所，合肥230031)摘要：从大豆根围土样中筛选得到对大豆疫霉有较好拮抗作用的生防菌株4株，经平板测定，生防菌株BY-

2、2的防效最好，达79．o0％；对其不同发酵时间的代谢液活性进行测定，发现72h代谢液的抑制效果最佳，达到40．93％BY-2代谢液能显著抑制大豆疫霉游动孢子萌发，抑制效果为39．89％～57．71％；其挥发性物质能显著抑制疫霉菌丝的生长，抑制效果为81．70％～91．94％。离体和盆栽试验结果表明，BY-2菌液处理对大豆疫病具较好的防治效果，离体叶片防效和盆栽防效分别为63_38％和75．01％。此外，用BY-2活菌液灌根大豆幼苗的鲜重、干重、根长和茎长显著增加，表明生防菌株BY-2能促进大豆植株的生长。关键词：生防细菌；大豆疫霉；生防效果；促生作用中图分类号：$435

3、．651文献标识码：A文章编号：1672—352X(2012)05—0677—05ScreeningandbiocontroleffectsofantagonisticbacteriaagainstPhytophthorasojaeYANGGuang—hong，GAOZhi．moulCAOShunlJIANGTao，WANGTao2，，(1．SchoolofPlantProtection，AnhuiAgriculturalUniversity,Hefei230036；2．InstituteofPlantProtection，AnhuiAcademyofAgricultu

4、ralSciences，Hefei230031)Abstract：Fourbacterialstrainswereisolatedfromthesoilinrhizosphereofsoybcanplants．AntagonistictestsshowedthatBY-2stronglyrestrictedthegrowthof1，tophthorasojae．Inthedual—culturetestinplates，theresultsshowedthatthebacterialcultureofstrainBY-2significantlyinhibitedthe

5、hyphalgrowthofPsojae,withahighinhibitionrateof79．0O％．TheresultsalsoshowedthattheinhibitionactivityoffermentationliguidagainstBY_2variedwithtime．andtheinhibitionofmetabolicliquidat72htreatmentagainstPsojaewas40．93％，beingthehighest．Themetabolicliquidcouldevidentlyinhibitgerminationofzoospo

6、res，withtheinhibitoryratesfrom39．89％to57．71％．ThevolatilesfromBY-2couldevidentlyinhibithyphalgrowthofPsojae，withtheinhibitoryratesfrom81．70％tO91．94％．Detached1eavesandpotexperimentsdemonstratedthatthebacteria1ofBY-2hadsignificantcontrolonsoybeanblight．withthecontroleffectivenessbeing63．38％

7、and75．01％，respectively．Inaddition，thefleshweightanddryweightwereallincreasedsignificantlyandthegrowthofsoybeanwaspromotedafterrootaffusedwithBY2bacterialsuspension．Keywords：antagonisticbacteria；Phytophthorasojae；biologicalcontrol；growthpromoting由大豆疫霉(PtophthorasojaeKaufma