大豆低聚肽对中波紫外线诱导的光老化小鼠皮肤的保护作用-论文.pdf

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1、Feb2014，Vol·28，No·21CHIliJo碱BR性m病VB学IIB汆RBD芯LIlI19大豆低聚肽对中波紫外线诱导的光老化小鼠皮肤的保护作用马立文，赵宏伟，周炳荣，尹智，骆丹，吴志韵，邹士玉[摘要]目的探讨大豆低聚肽(SOP)对中波紫外线诱导的BALB／c小鼠皮肤光老化的保护作用。方法BALB／c小鼠背部皮肤剃毛后予中波紫外线照射，构建小鼠皮肤光老化模型。随机分为各给药组，行UVB照射后分别外用不同浓度的SOP。组织切片、HE染色观察皮肤结构改变情况并检测表、真皮厚度；Masson染色观察胶原纤维改变情况并测定其胶原含量；试剂盒测定皮肤组织中超氧化物

2、歧化酶(SOD)、谷胱甘肤过氧化物酶(GSH-Px)活性和丙二醛(MDA)、羟脯氨酸(HYP)含量。结果各SOP外用组表现出不同程度的光保护效果，与模型组比较，SOP中剂量组可显著降低表皮厚度，减缓真皮胶原纤维降解，降低MDA含量，提高SOD，GSH．Px活性及HYP含量(P均<0．01)。结论SOP能有效提高小鼠抗氧化能力，对UVB所致BALB／c小鼠皮肤光老化具有保护作用。[关键词]大豆低聚肽；光老化；中波紫外线；BALB／c小鼠[中图分类号]R758．1[文献标识码]A[文章编号]1001—7089(2014)02—0119—04ProtectiveEfe

3、ctsofSoybeanOligopeptideonUVB-inducedSkinPhotoaginginMiceMALi—wen，ZHAOHong．wei，ZHOUBing—rong，YINZhi，LUODan，WUZhi．yun，ZOUShi．yu(1．DepartmentofDermatology，FirstAffiliatedHospitalofNanjingMedicalUniversity，Nanjing210029，China；2．InfinitusCompanyLtd，Guangzhou510000，China)[Correspondingaut

4、hor]LUODan，E—mail：daniluo2013@njmu．edu．cn[Abstract]ObjectiveTostudytheprotectiveeffectsofsoybcanoligopeptide(SOP)onUVB—inducedskinphotoagingofBALB／cmice．MethodsBALB／cmicewereirradiatedbyUVBforalongtimeontheshavedback．EachgroupwassmearedwithdifferentconcentrationofSOPafterUVBirradiati

5、on．HEstainingwasusedtoobservethehistologyofskinchangeandmeasurethethicknessofepidermisanddermis．Massonstainingwasusedtoobservethecollagenchangesandmeasurecollagencontent．Thesuperoxidedismutase(SOD)andglutathionperoxidase(GSH-Px)ativities，ma1ondialdehyde(MDA)andhydroxyproline(HYP)cont

6、entsweredetectedbyassaykit．ResultsThegroupswhichusedSOPshoweddifferentdegreesofprotectiveeffect0nphotoag·ing．Comparedwiththecontrolgroup，theepidermalthicknesswasreducedsignificantly，thedermalcollagendegradationwassloweddownsignificantly，andthecontentoftheMDAwasdecreasedsignificantlya

7、ndtheactivitiesofSOD，GSH—PxandthecontentoftheHYPwereincreasedsignificantly(all尸<0．01)inthegroupwhichusedmiddledosesofSOP．ConclusionSOPhasaprotectiveeffectontheskinphotoagingofBALB／cmiceviaincreasingtheantioxidationcapacity．[Keywords]Soybeanoligopeptide；UltravioletBradiation；Photoagin

8、g；BALB／cmice