大理单刺仙人掌提取物抑菌效果及抑菌机理的初步探讨.pdf

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1、大理学院学报第l3卷第8期2014年8月jouRNALOFDALIUNIVERSITYVo1．13No．8Aug．2014[DOI]10．3969／j．issn．1672-2345．2014．08．005大理单刺仙人掌提取物抑菌效果及抑菌机理的初步探讨黄媛，张鹏程，杨康，陈冬菊，刘文英，张雷(大理学院基础医学院，云南大理671000)[摘要]目的：探讨大理地区单刺仙人掌乙醇提取物和超声波提取物的体外抑菌效果及其抑菌机制。方法：以乙醇提取法和超声波提取法提取仙人掌原液，用K—B法分别测仙人掌提取物对标准株金黄色葡萄球菌、大肠埃希菌和白色念珠菌标准株的抑菌效果，以常量稀释法检测其最低

2、抑菌浓度，并以抑菌曲线初步探讨其抑菌机制。结果：两种方法提取的仙人掌原液对实验菌株均有抑制作用，乙醇提取物的最低抑菌浓度小于超声波提取物。抑菌曲线表明，乙醇提取物原液在金黄色葡萄球菌生长的9h内均表现出抑制作用。结论：大理地区的单刺仙人掌两种提取物对本实验的革兰阳性菌和革兰阴性菌及真菌均有抑制作用，其中乙醇提取物的抑菌效果较超声波提取物好。本研究可为仙人掌提取物的进一步研究和应用打下理论基础。[关键词]单刺仙人掌提取物；K—B法；最低抑菌浓度(MIC)；抑菌曲线；抑菌效果；抑菌机理[中图分类号]$682．33[文献标志码]A[文章编号]1672—2345(2014)08—0015

3、—03PreliminaryStudyontheAntibacterialEfectsandMechanismofOpuntiamonacanthaExtractsinDaliHUANGYuan，ZHANGPengcheng，YANGKang，CHENDongju，LIUWenying，ZHANGLei(Pre—clinicalCollege，DaliUniversity，Dali，Yunnan671000，China)[Abstract]Objective：Tostudytheantibacterialeffectsandmechanisminvitrooftheethanol

4、extractsandultrasonicextractsofopunticmonacanthainDali．Methods：EthanolextractionandultrasonicextractionwererespectivelyappliedtothepreparationofprimaryextractsofOpuntiamonacantha，andantibacterialeffectsonexperimentalstrainsStaphylococcusaureus，EscherichiacoliandCandidaalbicansweredetectedbyus

5、ingK—Bmethod．Theminimuminhibitoryconcentrations(MIC)weremeasuredbythemacrodilutionmethod，andtheantibacterialmechanismwaspreliminarilyexploredbytheantibacterialcurve．Results：Thereareinhibitoryeffectsintwokindsofprimaryextractsonexperimentalstrains．TheMICofethanolextractswerelowerthanthatofultr

6、asonicextracts．AntibacterialcurvedemonstratedthattheethanolextractsofOpunticmonacanthahadantibacterialeffectsonStaphylococcusaureuswithin9hoursofgrowth．Conclusion：TwokindsofextractsofOpunticmonacanthainDaliwereabletoinhibitthegrowthofexperimentalstrainsincludinggram——negativeandgram—‘positive

7、bacteriaandfungi；antimicrobialeffectsofethanolextractswerebetterthanthoseofultrasonicextracts．ThisstudycouldprovideatheoreticbasisforfurtherresearchonandapplicationoftheextractsofOpuntiamonacantha．[Keywords]Opuntiamonacanthaextracts；K—Bmethod