低温胁迫对嫁接西瓜耐冷性和活性氧清除系统的影响

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1、应用生态学报2004年4月第15卷第4期CHINESEJOURNALOFAPPLIEDECOL(_)GY，Apr．2004，15(4)：659～662低温胁迫对嫁接西瓜耐冷性和活性氧清除系统的影响*刘慧英L朱祝军¨吕国华(浙江大学蔬菜研究所，杭州310029；新疆石河子大学园林系，石河子832003)【摘要】研究了西瓜实生苗和以黑籽南瓜、超丰F1为砧木的嫁接苗的耐冷性及活性氧清除系统的差异．结果表明，低温胁迫下，嫁接苗的耐冷性明显高于实生苗，表现为以黑籽南瓜为砧木的嫁接苗的耐冷性>以超丰F1为砧

2、木的嫁接苗>实生苗．此外嫁接苗和实生苗均表现为叶片中叶绿素含量下降，丙二醛(MDA)含量上升，非酶促抗氧化剂抗坏血酸(A)、谷胱甘肽(GSH)含量和抗氧化酶超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(AsA．POD)、脱氢抗坏血酸还原酶(DR)活性下降，说明低温逆境降低了植物体防御活性氧有关的酶促和非酶促保护系统能力，提高了体内自由基浓度，加剧了膜脂过氧化．嫁接苗的活性氧清除能力均高于自根苗，且嫁接苗中耐冷性越强的活性氧清除能力越高。说明西瓜嫁接后耐冷性的提高是与植物体内活性氧清除系统中抗氧化

3、剂含量和抗氧化酶活性提高有关．关键词西瓜嫁接苗耐冷性活性氧清除系统文章编号1001—9332(2004)04—0659—04中图分类号$561，Q948．1122文献标识码AEffectoflowtemperaturestressonchillingtoleranceandprotectivesystemagainstactiveoxygenofgraftedwa·termelon．LIUHuiying“，ZHUZhujun，LUGuohuaz(InstituteofVegetableScien

4、ce，ZhejiangUniversity。Hangzhou310029，China；DepartmentofHorticulture&Forestry，ShiheziLiw，Shihezi832003，China)．-Chin．J．App1．Eco1．，2004，15(4)：659～662．Thispaperstudiedthechillingtoleranceandtheactivityofprotectivesystemagainstactiveoxygenofown．rootedandg

5、raftedwatermelonseedlings．TheresultSshowedthatunderlowtemperaturestress，thechillingtoleranceofthewatermelonseedlingsgraftedonfigleafwashigherthanthatoftheseedlingsgraftedonChaofengF1，andthatoftheown．rootedseedlingsWasthelowest．Thedecreaseofchlorophyl

6、l，ascorbateandGSHcontentsandSU．peroxidedismutase，ascorbateperoxidaseanddehydroascorbatereductaseactivitiesaswellastheincreaseofMDAcontentintheleavesofbothown-rootedandgraftedwatermelonseedlingsindicatedaninjuryontheprotectivesystemagainstactiveoxygen

7、，andinducedlipidperoxidationunderlowtemperaturestress．However，theactivityofprotectivesystemagainstactiveoxygenWashigheringraftedwatermelonseedlingsthaninown．rootedwater．melonseedlings．Theactivityofprotectivesystemagainstactiveoxygeninfigleafgourd-gra

8、ftedwatermelonseedlingswithstrongerchillingtoleranceWashigherthanthatofChaofengF1．graftedwatermelonseedlingswithweakerchillingtolerance．ItcouldbeconcludedthattheenhancementofchillingtoleranceingraftedwatermelonseedlingswasrelatedtOtheincreaseofantiox