mate_plate酵母双杂交文库构建中文说明书(clontech)

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1、“Mate&Plate™”文库构建系统说明书PT4085-1(PR013451)Cat.No.630490PublishedFebruary2010“Mate&Plate™”文库构建系统说明书目录I.介绍&操作流程..................................................................................................4II.组份列表.................................................

2、...........................................................5III.附加产品推荐...................................................................................................7IV.缩写列表.....................................................................................

3、......................8V.宿主菌株信息....................................................................................................9VI.对照实验..............................................................….……………………………………10A.总论.......................................

4、.........................................................……………….10B.操作:对照同源重组克隆的实验操作………………………………………………………....10VII.cDNA文库构建.............................................................................................11A.操作:第一链cDNA合成..............................

5、...................................................11B.操作:长距离PCR(LD-PCR)扩增cDNA............................................................13C.操作:CHROMASPIN™TE-400纯化dscDNA................................................14VIII.双杂交文库构建..............................

6、............................................................16A.酵母双杂交文库构建&筛选总论...................................................................16B.操作:制作Mate&Plate文库.........................................................................16IX.参考文献..............

7、...........................................................................................18X.疑难解答指南..................................................................................................19附录A:文库滴度测定...................................................

8、......................................24附录B:质粒信息................................................................................................22附录C:酵母生长培养基&补充物.....