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1、·48·JCAM.Feb,2010,Vol.26,NO.02针刺对实验性脑出血大鼠脑组织PAR-13表达的影响11△122王珑,邹伟,李丹,孔莹,迟庆滨(1.黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040;2.黑龙江中医药大学,黑龙江哈尔滨150040)摘要:目的:探讨急性脑出血后凝血酶受体-1(PAR-1)的动态表达及针刺的干预作用。方法:选取健康雄性Wistar大鼠96只,随机分为模型组、针刺组、西药组3组。每组又分为6h、24h、48h、72h和168h5个时相点,每个时相点6只大鼠,另设空白对照组6只大鼠。制备脑出血大鼠模型,采用West2ern免疫印迹技术观察不同时相点头穴
2、针刺治疗对脑出血大鼠脑组织PAR-1蛋白表达的影响。结果:针刺组大鼠脑内PAR-1蛋白表达在不同时相点均有显著下调,与模型组比较均有显著性差异(P<0.05),具有统计学意义。结论:下调脑内PAR-1蛋白表达可能是针刺治疗脑出血获效的一个重要机制,针刺可能通过下调脑出血血肿周围脑组织内PAR-1蛋白表达,减轻凝血酶所造成的毒性反应,从而达到防治脑出血后继发性脑损伤的作用。关键词:针刺;脑出血;凝血酶受体-1中图分类号:R24616文献标识码:A文章编号:1005-0779(2010)02-0048-03EffectofacupunctureontheexpressionofPar-1inth
3、ebraintissuearoundhematomaoftheratswithintercerebralhemorrhage11△2122WangLong,ZouWei,LiuFang,LiDan,KongYing,ChiQing-bin(1.SectionofAcupuncture&Moxibustion,FirstHospitalAffiliatedtoHeilongjiangUniversityofTraditionalChinesemedicine,Harbin150040,China;2.Departmentofcadre,firsthospitalofharbin150010,C
4、hina;3.HeilongjiangUniversityofTraditionalChinesemedicine,Harbin150040,China)Abstract:Objective:TostudythedynamicexpressionofPar-1afteracuteintercerebralhemorrhage(ICH)inratsandtheinfluenceofacupunctureonit.Methods:The96healthyWistarmaleratswereselected.Theratsweredividedinto3groupsrandomly:modelgr
5、oup,pinprickgroup,piracetamgroup,30ratsineachgroup.Eachgroupwasdividedintofivetimephasepointof6hours,1day,2days,3days,7daysagain,6ratsineachgroup.ExperimentICHmodelwasmadebyinjectionantologousbloodusingstereotacticmethod.Another6ratswereassignedasthematchedblankgroup.WeobservatedtheexpressionofPar-
6、1oftheintercerebralhem2orrageratwiththemethodsofwestern-blot.Results:TheexpressionsofPar-1inpinprickgroupsignificantlydown-regulatedthanthoseingroupsofmodelandpiracetam(P<0.05).Conclusion:Acupuncturemayeffec2tivelyinhibittheexpressionofPAR-1inbraintissueoftheratwithICHtorelievethelesionfromtheimmun
7、o2logicinflammationafteracuteICH.ThismaybeoneoftheimportantmolecularmechanismsofacupunctureonICH.Keywords:Acupuncture;Intercerebralhemorrage;PAR-1脑出血是急性脑血管疾病中的急危重症,其发病3基金项目:国家自然科学基金项目,30772840;黑龙江省中医局科研率、死亡
