镰形棘豆提取物抗肝癌的体内外活性研究-论文.pdf

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1、Availableonlineat1^n^n^，．sciencedirect．com∞Chinese糯ScienceDirectJournalofNaturalMedicinesChineseJournalofNaturalMedicines2013，l1(5)：0519-0524一doi：10．3724／SEJ．1009．2013．00519AntitumoreffectsoftwoextractsfromOxytropisfalcataonhepat0cellularcarcinomainvitroandinvivoYANGGuang—MinglaYANRu2a，WANGZhao

2、—Xian3，ZHANGFang—Fang，PANYang'．CAIBao—ChangKeyLaboratoryofStateAdministrationofTraditionalChineseMedicineforStandardizationofChineseMedicineProcessing＆JiangsuKeyLaboratoryofChineseMedicineProcessing，NanjingUniversityofChineseMedicine，Nanjing210023，China：UniversityofMacau，Macau，China,"SchoolofPh

3、armac~ChinaPharmaceuticalUniversity,Nanjing210009,China；LaboratoryofMedicalFungiandPhyto．Biotech，NanjingUniversityofChineseMedicine，Nanjing210023，ChinaAvailableonline20Sept．2013『ABSTRACT1AIMS：ToinvestigatetheantitumoreffectsofextractsfromOxytropisfalcataonhumanhepatocellularcarcinomaSMMC一7721ce

4、llsinvitroandintransplantedmurineH22tumorsinvivo．METHoDS：Cellproliferation．cellcycledistributionandapoptosisinSMMC．7721cellsweredeterminedandtumorgrowthinhibitioninH22tumorswasinvestigated．Cellcycledistributionwasanalyzedbyflowcytometrywithpropidiumiodide(PI)andAnnexinV．FITC，PIdoublestaining．RE

5、SULTS：MTTassayrevealedthatessentialoilandflavonoidsofO．falcata(namedEOOFandFOF、inhibitedproliferationofSMMC一7721cellsinadose—dependentmanner．TheIC50valueofEOOFandFOFwere0．115and0．097mg．mL～，respectively．CellcyclewasarrestedatGphase．andinductionofapoptosisoccu~edinSMMC一7721cellswhensubjectedtoFOF

6、．G『rowthinhibitioninH22solidtumorstransplantedmicewassignificantlypronouncedafterbeingtreatedwimF0F．andtheinhibitionratiowere56．1％and70．8％attheconcentrationof30and60mg·k2～．CoNCLUSION：TheresultssuggestthatFOFpromotesapoptosisinSMMC一7721cellsandinhibitsH22tumorgrowth，resultinginapotentialantitumo

7、teffectonhepaticcancer．[KEYW0RDS1Antitumor；OxytropisfaIcata；Apoptosis；SMMC一7721：Murinehepatoma22；Proliferation【CLCNumber】R965【Documentcode】A【ArticleID】1672—3651(2013)05—0519—06desperatelyneededinthetreatmentoflivercancerto1IntrOdu