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1、·300·ChineseJReparativeandReconstructiveSurgery,2002,Vol16(5)聚磷酸钙纤维体内降解及组织相容性实验#赵琳孙正义【摘要】目的研究人工合成聚磷酸钙纤维体内降解及组织相容性,为肌腱组织工程材料研究提供部分实验依据。方法聚磷酸钙纤维束和对照材料碳纤维在20只SD大鼠肢体对称部位的皮下、肌肉、肌腱内植入。术后1个月观察实验鼠的日常活动;术后当日,4、8、12、16及20周行X线片检查;术后4、8、12、16及20周取出材料,行肉眼观察、组织切片,HE染色。结果X线片及组织切片证实聚磷酸钙纤维在肌肉内约16周完全降解,肌腱、皮下约20周完全降
2、解,降解产物无局部沉积现象;而对照碳纤维在实验中未观察到明显降解。聚磷酸钙纤维周围组织内淋巴细胞及巨噬细胞明显少于碳纤维周围,且与周围增生组织相融合程度亦优于碳纤维。结论聚磷酸钙纤维在体内16～20周可完全降解,组织相容性优于碳纤维。【关键词】聚磷酸钙纤维体内降解组织相容性EXPERIMENTOFHISTOCOMPATIBILITYANDDEGRADATIONINVIVOOFARTIFICIALMATERIALCALCI-UMPOLYPHOSPHATEFIBERöZHAOLin,SUNZheng2yi.OrthopedicInstitute,LanzhouMedicalCollege.La
3、nzhouGansu,P.R.China730030【Abstract】ObjectiveTostudythehistocompatibilityanddegradationinvivoofanewartificialmaterial,calci2umpolyphosphatefiber(CPPF),andtoprovidesomeexperimentalbasisforfurtherstudyintendontissueengineering.MethodsCPPFandcarbonfiber(CF)ascontrolmaterialwereimplantedinsymmetrypart
4、insubcutaneouslayer,muscleandAchillestendonof20SDrats.Thedayofoperation,the4th,8th,12th,16th,and20thweeksafteropera2tion,X2rayexaminationwasperformedtodetectthedensitychangeofmaterials.Localtissueandmaterialswereobservedgrossly,andpathologicalexaminationwasmadewithHEstaining4,8,12,16weeksafteroper
5、ation.ResultsCPPFdegradedcompletelywithin16weeksinmuscle,andin20weeksafterimplantationinAchillestendonandsub2cutaneouslayeraccordingtoX2rayandpathologicalexamination.Nocalciumphosphatecrystaldepositwasobservedinlocaltissue.NoobviouslydegradationofCFwasfoundwithin20weeks.Localinfiltrationoflymphocy
6、tesandmacrophagocytesaroundCPPFweremuchfewerthanthatofCF.CPPFcombinedcompactlywithsurroundinghyper2plastictissue.ConclusionCPPFdegradethoroughlyfrom16to20weekswithoutsedimentofcrystalofcalciumphos2phateinvivo.CPPFhasgoodhistocompatibilityandcanbeusedasascaffoldmaterialoftissueengineering.【Keywords
7、】CalciumpolyphosphatefiberDegradationinvivoHistocompatibility肌腱组织结构及功能的特殊性,对肌腱组织工程CPPF由兰州铁道学院生物材料实验室提供若干研究的细胞支架材料提出了特殊要求,目前研究较多束,长10cm,经去污粉水浸泡、洗涤1小时,自来水,的碳纤维(carbonfiber,CF)、人发、聚乳酸等材蒸馏水反复漂洗数次,75%酒精浸泡30分钟,无菌生[1～3]
