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1、检验医学2004年第19卷第4期LaboratoryMedicine2004,Vol19.No4.·341·文章编号:100122087(2004)0420341203中图分类号:R446.62文献标识码:A抗核小体抗体与系统性红斑狼疮肾炎相关性研究陈瀑,严立(重庆医科大学附属第一医院检验科,重庆400016)摘要:目的探讨血清抗核小体抗体(ANuA)的检测在狼疮性肾炎(LN)患者中的意义。方法采用酶联免疫吸附试验(ELISA)检测35例LN和12例系统性红斑狼疮(SLE)无肾炎患者血清ANuA,并同时进行抗双链DNA(抗dsDNA)和抗Sm抗体检测。结果LN组与SLE无肾炎组患者ANuA
2、阳性率和抗dsDNA阳性率差异有显著性(P<0.001、P<0.05),而抗Sm抗体阳性率差异无显著性(P>0.05)。在LN组中,ANuA阳性率高于抗dsDNA(P<0.05),在SLE无肾炎组中,两者差异无显著性(P>0.05)。14例尿蛋白<3.5g/24h的LN患者ANuA阳性率高于抗dsDNA(P<0.05),而21例尿蛋白≥3.5g/24h的LN患者两者差异无显著性(P>0.05)。LN组患者血清ANuA含量明显高于SLE无肾炎组。结论血清ANuA的检测对于LN的诊断具有重要意义。关键词:抗核小体抗体;酶联免疫吸附试验;系统性红斑狼疮;狼疮性肾炎Studyontherelati
3、onshipbetweenanti2nucleosomeantibodyandlupusnephritisCHENPu,YANLi.Depart2mentofClinicalLaboratory,theFirstHospital,ChongqingMedicalUniversity,Chongqing400016,ChinaAbstract:ObjectiveTostudytheclinicalsignificanceofdeterminingserumanti2nucleosomeantibody(ANuA)inlupusnephritis(LN)patients.MethodsANuA
4、weredetectedbyELISAintheserumof35LNpatientsand12sys2temiclupuserythematosus(SLE)patientswithoutnephritis.Atthesametime,anti2dsDNAandanti2Smweredetect2ed.ResultsThepositiverateofANuAandanti2dsDNAinLNweresignificantlydifferentfromthatinSLEwithoutnephritis(P<0.001,P<0.05),andthepositiverateofanti2Smh
5、adnosignificantdifference(P>0.05).Thepos2itiverateofANuAshowedsignificantlyhigherthanthatofanti2dsDNAintheLNpatients(P<0.05),andtherewasnosignificantdifferenceinSLEwithoutnephritis(P>0.05).ThepositiverateofANuAshowedsignificantlyhigherthanthatofanti2dsDNAin14nephritispatientswithurineprotein<3.5g/
6、24h(P<0.05),andtherewasnosignifi2cantdifferencein21nephritispatientswithurineprotein≥3.5g/24h(P>0.05).LNpatientsshowedsignificantlyhigherserumlevelsofANuAcomparedwithSLEwithoutnephritis(P<0.05).ConclusionsDeterminationofserumANuAisimportanttomonitorANuAinLNpatients.Keywords:Anti2nucleosomeantibodi
7、es;ELISA;Systemiclupuserythematosus;Lupusnephritis系统性红斑狼疮(systemiclupuserythemato2材料和方法sus,SLE)及狼疮性肾炎(lupusnephritis,LN)的特点是静止期和活动期交替出现。如何判断疾病一、标本来源和收集的活动度及肾脏是否有活动性炎症,特别是对于临床确诊的SLE患者47例,其中LN患者LN的判断、免疫抑制剂的及时应用非常重要
