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1、中西医结合学报2005年9月第3卷第5期JChinIntegrMed,September2005,Vol.3,No.5·391·①疏肝理肺方防治应激条件下大鼠哮喘的作用机制研究1234童瑶,张宁霞,陈惠娟,倪力强(1.香港大学中医药学院,香港;2.上海中医药大学普陀医院康复科,上海200062;3.上海中医药大学基础医学院,上海201203;4.上海医药情报研究所,上海200063)[摘要]目的:探讨心理因素对哮喘发病的影响和调肝法调节应激、防治哮喘的作用机制。方法:用卵蛋白加束缚应激刺激建立应激条件下哮喘大鼠模型,采用放射免疫分析法、酶联免疫吸附法、原位杂交、免疫组化、光镜及电镜等方法,
2、检测各组大鼠血浆皮质酮、白细胞介素4、干扰素γ、海马糖皮质激素受体mRNA和下丘脑促肾上腺皮质激素释放激素(adrenocorticotropichormonecorticoliberin,CRH)阳性神经元,并观察肺和海马组织形态学的变化。结果:疏肝理肺方可明显降低血浆皮质酮水平,升高血浆干扰素γ,降低血浆白细胞介素4,减少下丘脑室旁核CRH阳性神经元,上调海马CA3区糖皮质激素受体mRNA的表达,减轻海马神经元的损伤,并可明显减轻应激条件下哮喘大鼠肺组织的病理改变。结论:疏肝理肺方通过抑制应激条件下哮喘大鼠下丘脑-垂体-肾上腺皮质轴功能的亢进及调节免疫系统的功能,从而起到防治哮喘的作用
3、。[关键词]应激;哮喘;疏肝理肺法[中图分类号]R562.65[文献标识码]A[文章编号]1672-1977(2005)05-0391-06ProtectiveandtherapeuticeffectsofShuganLifeiRecipeonratswithasthmaunderstressandthemechanisms1233TONGYao,ZHANGNing-Xia,CHENHui-Juan,NILi-Qiang(1.SchoolofChineseMedicine,UniversityofHongKong,HongKong,China;2.DepartmentofRehabilit
4、ation,PutuoHospital,ShanghaiUniversityofTraditionalChineseMedicine,Shanghai200062,China;3.BasicMedicalCollege,ShanghaiUniversityofTraditionalChineseMedicine,Shanghai,201203,China;4.ShanghaiInstituteofMedicalInformation,Shanghai200063,China)ABSTRACTObjective:Toexploretheinfluenceofpsychologicalfact
5、oronasthma,theprotectiveandthera-peuticeffectsofShuganLifeiRecipeonratswithasthmaunderstressandthemechanisms.Methods:Allergyandstress-inducedasthmamodelswereestablishedinratsbygivingovalbuminandrestrainingstress.Radio-immunoassay,enzymelinkedimmunosorbentassay,insituhybridizationandimmunohistroche
6、micalmethodwereusedtodetectplasmacorticosterone,IL-4,IFN-γ,expressionofglucocorticoidreceptor(GR)mRNAinhippocampusandadrenocorticotropichormonecorticoliberin(CRH)positveneuroninhypothalamus.Opticalandelectronmicroscopeswereusedtoobservethemorphologicalchangesofpulmonaryhilarandhippocam-paltissues.
7、Results:ShuganLifeiRecipe(SGLFR)couldreduceplasmacorticosterone,decreaseCRHposi-tiveneuronsinparaventricularnucleusofhypothalamus,up-regulatetheexpressionofGRmRNAinhippocam-pusandreducetheinjuryofhippocampalneuro
