CELLULASE from TRICHODERMA LONGIBRACHIATUM-纤维素酶(源自木霉菌).pdf

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1、CELLULASEfromTRICHODERMALONGIBRACHIATUMPreparedatthe39thJECFA(1992),publishedinFNP52Add1(1992)supersedingspecificationspreparedatthe31stJECFA(1987),publishedinFNP38(1988)inFNP52(1992)underthenameCellulasefromTrichodermareesei.AnADI'notspecified'wasestablishedatthe39thJECFA(1992)SOURCESCommerci

2、alenzymepreparationsareproducedextracellularlybythecontrolledfermentationofTricodermalongibrachiatumandisolatedfromthemedium.ThenameoftheorganismusedpreviouslywasTrichodermareesei.In1986,theInternationalCommissionontheTaxonomyofFungi(ICTF)recommendeduseofthenameTrichodermalongibrachiatum.Activ

3、eprinciples1.Cellulase(endo-1,4-ß-glucanase)2.Exo-1,4-ß-D-glucosidase(glucan-1,4-ß-glucosidase)3.Exo-cellobiohydrolase(cellulose1,4-ß-cellobiosidase)4.ß-glucanaseSystematicnamesand1.1,4-(1,3;1,4)-ß-D-Glucan-4-glucanohydrolase(EC3.2.1.4)numbers2.1,4-ß-D-Glucosideglucohydrolase(EC3.2.1.74)3.1,4-

4、ß-D-Glucancellobiohydrolase(EC3.2.1.91)4.1,3-(1,3;1,4)-ß-D-glucan-3(4)-glucanohydrolase(EC3.2.1.6)ReactionscatalyzedTheenzymepreparationshydrolyze1,4-ß-glucanlinkagesinsuchpolysaccharidesascellulose,yieldingß-dextrins.SecondaryenzymeAmyloglucosidase(EC3.2.1.3);CellobiaseactivitiesXylanase(EC3.

5、2.1.32);ProteinaseHemicellulase;Lipase(ED3.1.1.3)Pectinase(EC3.2.1.15)DESCRIPTIONOff-whitetotanamorphouspowders,orliquidsthatmaybedispersedinfood-gradediluentsandcarriers;solubleinwaterbutpracticallyinsolubleinethanol,chloroformandetherFUNCTIONALUSESEnzymepreparationUsedinthepreparationoffruit

6、juices,wine,beerandvegetableoilsGENERALMustconformtotheGeneralSpecificationsforEnzymePreparationsusedinSPECIFICATIONSFoodProcessing(seeVolumeIntroduction)CHARACTERISTICSIDENTIFICATIONCellulaseactivity(Vol.4)Thesampleshowscellulaseactivity