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1、http://www.paper.edu.cnTheeffectofearlyauditorydeprivationontheage-dependentexpressionpatternofNR2BmRNAinratauditorycortexCaixiaBi,YileiCui,YutingMao,JinghongXu,FengXu,JipingZhang,XindeSun*ResearchCentreforBrainScience,EastChinaNormalUniversity,Shanghai200062,
2、ChinaAbstractUsinginsituhybridization(ISH),presentresearchminutelyinvestigatedtheage-dependentexpressionpatternofNR2BmRNAinratauditorycortex(AC)duringpostnataldevelopmentandtheeffectofearlyauditorydeprivation.Fortheratsinthenormalgroup,themRNAexpressionofNR2Bw
3、ashighestatbirth(postnatalday1),butdeclinedrapidlyuntillowlevelofadulthood,duringwhichtherewasarisingperiodwithatransientpeakonpostnatalday21(P21);fortheratsinthedeprivedgroup,thewholedeclining-as-agingtrendofNR2BmRNAexpressionwassimilartothatofnormalgroup,but
4、theexpressionlevelfromP15toP27weremuchlowerthanthatofnormalgroupwiththegreatestreductionfromP21toP23andthetransientpeakonP21disappeared.Inbothgroups,thedistributionpatternofNR2BmRNApositiveneuronsin6layersanddorsal,medialandventralsub-districtsofauditorycortex
5、wasalsoexamined.Thereisnosignificanteffectontheexpressionpatternbetweennormalanddeprivedgroup.Ourresultsindicatedthattheearlyauditorydeprivationdecreasedtheage-dependentexpressionpatternofNR2BmRNA,suggestingthatNR2Bmayplayanimportantroleinthedevelopmentalplast
6、icityofauditoryfunctioninrats.Keywords:AuditoryCortex;AuditoryDeprivation;Insituhybridization(ISH);NR2BmRNAExpression1.IntroductionDuringasensitiveperiodinpostnataldays,animalsshowaformofexperience-dependentdevelopmentplasticityincentralnervoussystem.Ithasbeen
7、proposedthattheN-methyl-D-asparate(NMDA)receptorplayacrucialroleduringthiscriticalperiod.Withage,thechangesofNMDARfunctionmayberelatedtothevarietyofthesubunitcomposition,whichincludetheNR2BandNR2A[30,40].Intheneocortex,theNMDAreceptoriscomposedofNR1andNR2subun
8、its.NR2AandNR2B,thetwoNR2subunitshaveattractedfocusesrespectively.SomeresearchproposedthatNR2BmightpromoteplasticitymorethandoestheNR2Asubunit[41,43,44].Nakanishihavedemonstratedth
