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1、Chapter1proteinstructureandfunctionproteinbiomolecularaminoacidpolaritynonpolarityhydrophobicityneutralityacidityalkalityglycine,Glyalanine,Alavaline,Valleucine,Leuisoleucine,Ilephenylalanine,Pheproline,Protryptophan,Trpserine,Sertyrosine,Tyrcysteine,Cysmeth
2、ionine,Metasparagine,Asnglutamine,Glnthreonine,Thrasparticacid,Aspglutamicacid,Glulysine,Lysarginine,Arghistidine,HisbasicaminoacidacidicaminoacidpolaraminoacidneutralaminoacidSpectrophotometryAbsorbanceSpectrumUltravioletinfraredabsorptionpeakwavelengthsolu
3、bilitysidechainaminogroupguanidiniumgroupimidazolylcarboxygroupisoelectricpoint,pIpeptidepolypeptidechainpeptidebondoligopeptidepolypeptideaminoterminalcarboxylterminalresidueninhydrin/triketohydrindenehydrateiminoacidglutathione,GSH12/12hydrosulfidegroup/su
4、lfhydrylprimarystructuresecondarystructuretertiarystructurequaternarystructureα-helixβ-pleatedsheetβ-turnrandomcoilzincfingerdomainpeptideunitmotifproteindenaturemolecularchaperoneallostericeffectsubunitpositivecooperativitynegativecooperativityrenaturationp
5、roteincoagulationninhydrinreactionbiuretreactiondialysissaltprecipitationelctrophoresischromatographyprostheticgroupprionproteindisulfidehydrogenbondhydrophobicinteractionelectrostaticinteractionVanderWaalsinteractionFibrousproteinConformationalchangeGlobula
6、rproteinProteinfoldIonicbondSidechaintetramerdimmermonomeroligomerdenaturationrenaturationQuestions:1.Canyougiveanexampletoelucidatetherelationshipbetweenproteinprimarystructureandfunction?b5E2RGbCAP2.Canyougiveanexampletoelucidatetheproteinallostericeffect?
7、p1EanqFDPwChapter2StructureandFunctionofNucleicAcidnucleotidenucleticaciddeoxyribonucleicacid,DNA12/12ribonucleicacid,RNAthechemicalcomponentbasebasepairbasepairingribosephosphatepurineadenine,Aguanine,Gpyrimidinecytosine,Curacil,Uthymine,Tribosedeoxyriboser
8、ibonucleosideribonucleotideglycosidicbondnucleosidemonophosphate,NMPnucleosidediphosphate,NDPnucleosidetriphosphate,NTPphosphodiesterlinkagepolydoxynucleotidepolynucleotidenitrogenousbasedoubleh
