欢迎来到天天文库
浏览记录
ID:52533855
大小:274.72 KB
页数:5页
时间:2020-03-28
《中药复方对尾吊大鼠心肌ATP酶、SDH和能荷值地影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、第17卷第5期航天医学与医学工程VOI.17NO.52004年10月SpaceMedicine&MedicaIEngineeringOct.2004·学术论著·*中药复方对尾吊大鼠心肌ATP酶、SDH和能荷值的影响王宝珍,李勇枝,辛冰牧,范全春,白桂娥(航天医学工程研究所,北京100094)摘要:目的观察中药复方对短期模拟失重(尾吊)大鼠心肌ATP酶、SDH和能荷值的影响。方法大鼠分对照组、尾吊组和中药组(中药+尾吊),每组10只。尾吊5d后,3组大鼠同时处死,取心肌测ATP酶和SDH,酶不同染色处理并定量测其光密度(OD),测心肌能荷值定量表示能
2、量储备。结果尾吊组心肌细胞SDH染色比中药、对照组增强,OD值升高,ATP酶明显增强和OD值显著升高,心肌能荷值减少,其顺序为尾吊组>对照组>中药组。结论中药组在尾吊时由于中药复方发挥了镇静、安神、活血化瘀等药理作用,使大鼠心肌SDH活性减弱,ATP酶活性明显降低,能耗减少。关键词:失重模拟;尾吊;中药学;复方;心肌;ATP酶;琥珀酸脱氢酶;能荷值中图法分类号:R852.22;R856.2文献标识码:A文章编号:1002-0837(2004)05-0326-04EffectsofChineseHerbCompoundonMyocardialSDH,
3、ATP-aseandEnergyReservesinTail-suspendedRats.WANGBao-zhen,LlYong-zhi,XlNBing-mu,FANOuan-chun,BAlGui-e.SpaceMedicine&MedicalEngineering,2004,17(5):326~329Abstract:ObjectiveToinvestgateeffectsofChineseherbcompoundonmyocardialSDH,ATP-aseandenergyreservesintail-suspendedrats.Metho
4、dMaleSDratswererandomlydividedintothreegroups(n=10each):(A)normalcontrolgroup;(B)tail-suspendedgroup;(C)tail-suspended+ChineseMedicalherbcompoundgroup.RatsingroupBandCweretail-suspended(-30)for5dtosimulateweightlessness.AllratsBweredicapitatedaftartheexperiment.MyocardialSDH(m
5、ethodofAndersonN-BT),ATP-ase(methodofWachsteinandMeisel)andenergyreserves(methodofHPLC)wereexamined.ResultComparedwithratsingroupsAandC,SDHactivityin-creased,SDHstainingdeepened,valueofODSDHenhanced,ATP-aseactivityandODvaluein-creasedandenhancedsigneficently(!<0.01)butvalueofm
6、yocardialenergyreservesdecreasedingroupBrats.ConclusionTail-suspensionstrengthenscompensatoryoxidationmetabolismofcardiacmuscleandactivatsSDHandATP-ase,atthesametimedecrease,myocardialenergyreserves.Normalaremaintainedthelowlevelofmetabolismbythemedicalherbcompoundusedwhichsho
7、wsaprotectingeffectsthroughmitigation,tranguilizationandreplenishmentofOi.Keywords:weightlessnesssimulation;tailsuspension;Chinesemateriamedica;compounds;my-ocardium;ATP-ase;SDH;energyreservesAddressreprintregueststo:WANGBao-zhen.lnstituteofSpaceMedico-Engineering,Beijing10009
8、4,China由于失重引起心血管脱适应,就是短期失重其代谢水平密切相关,可是关于短时间模拟失重返回后也常引起立位耐力下降,
此文档下载收益归作者所有