产枯草芽孢杆菌训实报告.ppt

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1、生化工艺实训报告Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.

2、2.0.0.Copyright2004-2011AsposePtyLtd.一产淀粉酶的枯草芽孢杆菌的优化1、产淀粉酶枯草芽孢杆菌斜面活化2、摇瓶发酵优化培养基及种子培养3、葡萄糖标准曲线的制备4、发酵参数的测定Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.

3、0.Copyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.1、产淀粉酶芽孢杆菌斜面活化实验材料菌种：从土壤中分离产淀粉酶的枯草芽孢杆菌配方：牛肉膏蛋白胨培养基蒸馏水400ml、牛肉膏2g、蛋白胨4g、氯化钠2g、琼脂0.2g、PH7.0~7.2试剂：牛肉膏、氯化钠、氢氧化钠溶液、蛋白胨、琼脂、盐酸溶液Evaluationonly.Cre

4、atedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011As

5、posePtyLtd.实验内容1、制备培养基：依照培养基组成稀释后定容400ml，分装与6个摇瓶中，50ml。剩余溶液分装与6个试管中，均10ml，并加0.2g琼脂。2、灭菌：高压灭菌锅121摄氏度0.11MPa20~30min3、斜面摆放：灭菌后的试管静止冷却至50摄氏度左右，将试管的液面及搁置的试管斜面长度不超过试管总长一半，凝固后待用。4、斜面活化：在超净工作台中将保藏的芽孢杆菌接种置已制好的斜面上划线。5、恒温培养：恒温培养箱37摄氏度过夜培养进行活化Evaluationonly.CreatedwithAspose.Slidesfor

6、.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.2、摇瓶发酵优化培养基实验

7、材料试剂：牛肉膏蛋白胨氯化钠淀粉葡萄糖仪器设备：分光光度仪、超净工作台、烧杯、摇瓶、电炉、斜面活化的菌种、牛肉膏蛋白胨液体培养基、玻璃棒等玻璃仪器实验步骤：Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposeP

8、tyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyri