山薯组培苗继代培养基配方筛选.pdf

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1、广西农业科学2010，41(8)：758—761GuangxiA鲥culturMSciences山薯组培苗继代培养基配方筛选严华兵h，龚明霞·b，董伟清h，1-朝阳la)闭志强h，李杨瑞1(1广西农业科学院a生物技术研究所；b蔬菜研究所，南宁530007)摘要：以山薯组培苗带腋芽茎段为外植体，以Ms为基本培养基，探讨植物生长调节剂6—8A(0．5、1．0、2．0rag／L)或TDZ(0．01、0．10、0．50、1．00、2．00、4．00mg／L)、不同培养方式和活性炭对其继代培养的影响。结果表明，添加不同浓度6一BA对山薯组培苗增殖系数、茎长及腋芽点芽球

2、的影响不明显，而随着TDZ浓度的升高，山薯组培苗增殖系数和茎长逐渐降低而芽球显著增加，茎段腋芽生长受到明显抑制。山薯组培苗较优的继代培养基为MS+I．0mg／L6-BA+0．1mg／LNAA+30g／L蔗糖+1．5g／L活性炭的液体培养基。液体培养基利于促进山薯组培苗增殖。其增殖系数、茎长、植株鲜重和干重显著高于固体培养基。活性炭的添加可以减少褐化，促进组培苗的增殖培养。此外，培养方式与活性炭交互作用对组培苗茎长、增殖系数、植株鲜重及干重无显著影响。关键词：山薯；继代培养；增殖系数；固体培养；液体培养；活性炭中图分类号：$632．103"．53文献标识码：A

3、文章编号：1002—8161(2010)08-0758-04Screeningthesubcultlmediumfor’—、。‘fordiiPrl"etscreeningthesubculturemedluretorDioscoreatbrdlJPrainetBurkplantletsrANHua-bingh，GONGMing-xia¨，DONGWei-qingh，BUZhao-yangh，BIZhi-qian91a9LIYang—rui1(aBiotechnologyResearchInstitute；bVegetableResearchInstitute

4、，lGuangxiAcademyofA画culturalSciences，Nanning530007，China)Abstract：TheshootswithaxillarybudsderivedfromD．fordii(Guihuai21plantletswereusedasexplantstoinvesti．gatetheeffectsofdifferentplantgrowthregulators，沱．，6一BA(0．5，1．0and2．0mg／LandTDZ(0．01，0．10，0．50，1．oo，2．00and4．00mg／L)inMSmedium

5、，culturemedia(solidorliquid)andactivatedcarbon(AC)onsubcuhureofplantlets．There．suitsshowedthat1．0metE6一BAsupplementedinMSbasalmediumhadnosignificanteffectsonproliferationcoefbcient．stemlengthandgemmulesderivedfromaxillarybuds．WiththeincreaseinTDZconcentrationinMSmedium．theprolifer-

6、ationcoefficient，stemlengthofproliferatedplantletsandgrowthofaxillarybudsinhibitedsignificantly，whilenumberofgemmuleincreased．1heliquidMSmedium+1．0mg／L6-BA+0．1mg／LNAA+30．0g／LSugar+1．5g／LACwasfoundtobet11ebestmediumforshootproliferation．Comparedtosemi—solidculture．theliquidculturepr

7、omotedshootproliferationsig-nificantly，proliferationcoefficient，stemlengthandfreshweiIghtanddryweightofsubcultureplantletswerealsofoundhigher．AdditionofACinMSmediumreducedthebrowningofexplantsandpromotedtheshootproliferation．Furthermore。theinteractionbetweenculturetypeandACinMSmedi

8、umhadnosignificanteffectso