壬基酚对中华大蟾蜍蝌蚪的毒性效应.pdf

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1、农业环境科学学报2010，29(6)：1086-1090JournalofAgro·EnvironmentScience壬基酚对中华大蟾蜍蝌蚪的毒性效应吕胡，张迎梅，杨峰，司万童(兰州大学生命科学学院，兰州730000)摘要：为评价水体中低浓度壬基酚(NP)对中华大蟾蜍(曰ufobufogargarizans)蝌蚪的毒性效应，采用室内饲养、测定方法，研究了NP在不同暴露时间(8、16、24、32d)，不同浓度(O．002、0．005、O．010mg·L一)条件下，对蝌蚪生长发育、过氧化氢酶(CAT)活性、丙二醛(MDA)含量和DNA损伤的影响。结果表明：暴露于0．

2、010mg·L-1NP的蝌蚪，其生长发育被极显著抑制(P

3、蝌蚪；毒性效应中图分类号：X503．225文献标志码：A文章编号：1672—2043(2010)06—1086—05ToxicEffectsofExposuretoNonylphenolonBufobufogargarizansTadpolesLVYue，ZHANGYing-mei，YANGFeng，StWan—tong(SchoolofLifeSciences，LanzhouUniversity，Lanzhou730000，China)Abstract：Therewerefewreportsonthetoxiceffectsofnonylphenol(NP)on

4、tadpoles．ThisresearchwasdesignedtOinvestigatethedevelop-ment，catalase(CAT)activity，contentofmalonyldialdehyde(MDA)andDNAdamageofaufobufogargarizanstadpolesexposedtolowcon-centrationofNP．ThetadpoleswereexposedtoNPconcentrationsof0．002，0．005and0．010mg·L—foraperiodof8，16，24and32d．Theresu

5、ltswereasfeUows：(1)ThedevelopmentoftadpolesWasinhibitedsignificantlyduringexposuret00．010mg·L。NP(P<0．叭)．(2)TheCATactivityWasinducedinitiallyandthendepressedtocontrollevelattheconcentrationsofO．002and0．005mg·L-1，whiletheCATactivityWasfirstinduced，andthendepressed，butinducedagainattheco

6、ncentrationof0．010mg·L。1(P<0．05)．(3)ThecontentofMDAincreasedgraduallyfrom24to32dafterexposureto0．002and0．005mg·L-1NP(19<0．05)。andthereexistedatime-effectrelationship．(4)Comparedwiththecontrolgroup，thelevelofDNAdamageincreasedtothetopvalueat16d(P<0．05)，displayingadose—effectrelationshi

7、p，butitde—creasedastheexposuretimeprolonging．Inconclusion．evenattheconcentrationlowerthantheirrigationstandard。NPstillinhibitedthede—velopmentoftadpoles,andresultedinoxidativeinjuryandDNAdamage．Keywords：nonylphenol；8ufobufogargarizam；tadpole；toxiceffect壬基酚(nonylphenol，NP)是非离子表面活性剂壬基酚聚

8、氧乙烯醚(