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《镉对中华稻蝗保护酶系统的毒性效应.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、农业环境科学学报2010,29(8):1448—1454JournalofAgro—EnvironmentScience镉对中华稻蝗保护酶系统的毒性效应李丽君1,郭亚平2,一,武文丽1,王跃2,马恩波2(1.山西省农业科学院土壤肥料研究所,太原030006;2.山西大学生物应用学研究所,太原030006;3.山西大学生命科学学院,太原030006)摘要:采用急性染毒方法研究cd2+胁迫对不同发育阶段中华稻蝗SOD、CAT、POD活性的影响。结果表明,在cd2+的作用下,不同发育阶段中华稻蝗体内SOD、CAT、POD活性均发生变化。SOD、CAT、POD3种酶对cd2+
2、有一定的耐受性:当cd2哝度在这3种酶的耐受范围内酶活性提高,反之酶活性降低。cd埘不同发育阶段虫体3种抗氧化酶的影响不同:3龄若虫SOD、POD活性最高;雌性成虫CAT活性最低,而3龄雄性若虫CAT活性最高。关键词:中华稻蝗;镉(Cd);超氧化物歧化酶(SOD);过氧化氢酶(CAT);过氧化物酶(POD)中图分类号:X503.223文献标志码:A文章编号:1672—2043(2010)08—1448—07ToxicEffectsofCd2+onProtectiveEnzymeSystemsinDifferentDevelopingStageofOxyachinens
3、is(Or-thopteraAcridoidae)LILi-junl,GUOYa—pin矛3,wuWen—lil,WANGYue2,MAEn—b02(1.InstituteofSoilScienceandFertilizer,ShanxiAcademyofAgriculturalSciences,Taiyuan030006,China;2.IstituteofAppliedBiology,ShanxiUniversity,Taiyuan030006,China;3.CollegeofLifeScience,ShanxiUniversity,Taiyuan030006,
4、China)Abstract:InordertoclarifythetoxicmechanismofCd“inOxyachinensis,thetoxiceffeetofCd2+ontlleactivitiesoftllreeantioxidanten—zymes,superoxidedismutase(SOD),catalase(CAT)andperoxidase(POD),indifferentdevelopingstageofO.chbwnsisinjectedwithCdweredetemfined.DifferentdevelopingstagesO.chi
5、nensiswereinjectedwithCd2+atdifferentconcentrations(0,50,100,150,200lng‘L。1).TheresultsshowedthattheeffectsofCdz+onSOD。CATandPODactivitiesweredifferentwithintheexperimentalgradient.Cd2+couldinducerisesoftlleactivitiesofSOD.PODandCATatsomecontents。andinducetlledeclineoftheactivitiesatoth
6、ercontents.Thereweresignificantdifferences(P<0.05)ofSOD,CATandPODactivitiesamongthetreatlnents(exceptforSODofnlalesatthefifth—stagenymphsandadultO.chiner蕊).7TheactivitiesofCATandPODprobablydependedonSODactivitytoeliminateoxygenspecies(ROS)underCd2+stressintheexperiment.SODandPODactiviti
7、eswerethehighestatthethirdinstarnymphs,andtheactivityofCATinfemaleO.chinensiswasthelowestatadults,andtheactivityofCATinmaleO.chinensiswasthehighestatthethirdinstarnymphs.TheseresultsindicatedthattheactivitiesofSOD.CATandPODinO.chinensismightactasindicativefunctionsforCd“contami
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