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1、目录摘要1关键词1Abstract1Keywords1引言11材料与方法21.1材料21.2实验方法21.3适应性指标与计算32结果与讨论42.1pH对大蟾蜍蝌蚪的毒性影响42.2大蟾蜍蝌蚪对pH的适应能力52.3pH变动对大蟾蜍蝌蚪适应能力的影响5参考文献75.1-9,,services,andmakethecitymoreattractive,strengtheningpublictransportinvestment,establishedasthebackboneoftheurbanrailtransit
2、multi-level,multi-functionalpublictransportsystem,thusprotectingtheregionalpositionandachieve大蟾蜍蝌蚪对水环境pH的适应性摘要：采用单因子实验法，研究水环境pH值对大蟾蜍(Bufogargarizans)蝌蚪的急性毒性效应，探讨适应蝌蚪存活和发育的最佳pH值范围，提高蝌蚪养殖效果。结果表明，pH在4.4~9.6对蝌蚪96h存活率没有影响，其急性毒性效应的24h、48h、72h和96h半致死值（LpH50），对应的碱性范围
3、分别为10.17、9.98、9.87及10.00，酸性范围分别为3.85、3.94、4.30及4.37。大蟾蜍蝌蚪对pH的适应范围为4.40～9.58，最高耐受限为10.48，最低耐受限为3.50。蝌蚪养殖的水环境pH宜控制在6.0～8.0。关键词：大蟾蜍；蝌蚪；pH；适应性TheAdaptabilityofTadpolesinBufogargarizanstothePHunderDifferentAquaticEnvironmentLIYu-hua(GansuAgriculturalUniversityScho
4、oloflifesciences,GansuLanzhou730070)Abstract:What the method of this experiment applied is the single factor test. In order to promote the cultivation of the Bufogargarizans, it made a study of the ph value of water environment on the acute toxicity of Bufoga
5、rgarizans,and also discussed the applicability of Bufogargarizans to the PH. The result showed thatPHhasnoeffectonsurvivalrateoftadpolesafter48hourslate,the24h,48h,72hand96hLpH50oftadpolesare10.17,9.98,9.87and10.00respectivelyintherangeofalkalinity,thereare3.
6、85,3.94,4.30and4.37respectivelyintheacidrange.TheadaptationrangeoftadpolestoPHis4.40～9.58,thehighestlimitationis10.48,thelowestlimitationis3.50.TheaquaticenvironmentPHforcultureoftadpolesshouldbecontrolledintherangeof6.0～8.0.Keywords:Bufogargarizans;Tadpole;p
7、H;adaptability引言大蟾蜍(Bufogargarizans)是一种具有较高药用价值的两栖类动物,分布于我国大部分地区,随着其生活环境的逐渐破坏,野生大蟾蜍的数量迅速下降,使得其养殖业也得到快速的发展[1]。蟾蜍全身都是宝，头、皮、舌、肝、胆、干蟾等均可入药。明代李时珍的《本草纲目》就有蟾蜍退热、祛湿、杀虫之功效。近代医学分析，蟾蜍含有多种药用功能。5.1-9,,services,andmakethecitymoreattractive,strengtheningpublictransportinves
8、tment,establishedasthebackboneoftheurbanrailtransitmulti-level,multi-functionalpublictransportsystem,thusprotectingtheregionalpositionandachieve用于驰名中外的“六神丸”、“六应丸”、“麝香保心丸”等数十种名贵中成药的原料，临床上用
