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《嗜水气单胞菌、爱德华氏菌灭活菌苗免疫保护及药物治疗试验.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、广西农业科学2010,41(3):266-269GuangxiAgriculturalSciences嗜水气单胞菌、爱德华氏菌灭活菌苗免疫保护及药物治疗试验邓显文,谢芝勋‘,刘加波,谢志勤,谢丽基,庞耀珊(广西兽医研究所,南宁530001)摘要:嗜水气单胞菌、爱德华氏菌培养物经甲醛灭活后,制成灭活油乳剂苗、灭活菌苗、饵料吸附型疫苗,免疫罗非鱼2周后,分别以嗜水气单胞菌、爱德华氏菌活菌液攻毒,结果显示,注射型疫苗均优于口服型,其中嗜水气单胞菌以腹腔注射0.1mL嗜水气单胞菌灭活菌苗+o.3mL生理盐水的效果最佳,免疫保护率为85.O%;而爱德华氏菌以腹
2、腔注射0.4m1.灭活油乳剂苗效果最佳,免疫保护率为90.O%。两者的药物治疗试验结果显示,以氟苯尼考、恩诺沙星、环丙沙星、氟哌酸治疗罗非鱼嗜水气单胞菌病、爱德华氏菌病均有一定效果,对减少发病率及死亡率、控制病情具有较好作用,其中以氟苯尼考的疗效最佳,其用量为20mg/kgH20。关键词:罗非鱼;嗜水气单胞菌;爱德华氏菌;灭活菌苗;免疫保护;药物治疗中图分类号:$965.125文献标识码:A文章编号:1002—8161(2010)03-0266-04Protectiveimmunityintilapiaagainstinactivatedbacter
3、inofAeromonashydrophilaandEdwardsiellaand£ontrollingeffects0IantlDacterlalmediClnesnJ●■·●ll●●DENGXian-wen,XIEZhi-xun‘,LIUJia-bo,XIEZhi-qin,XIEU-ji,PANGYao-shan惭an铲iVeterinaryResearchInstitute,Nanning530001,China)AI斌ract:ThecoloniesofAeromollB8hydrophilaandEdwardsiellastrainsWe】
4、l'eculturedonnutritionbrothmediumfor24hat28℃.thestrainsolutionWagcolletedandinactivatedwithformaldehydesolutionfor2daysforthepreparationofinactivatedoil叫ldiuvantvaccine,inactivatedbacterinandbait-absorbedvaccine.Thetilapiawasimmunizedwiththreeinactivatedbacterinfortwoweeksandin
5、jectedwithlivestrainsolutionofAeromon船hydmphilaandEdwardsiella.Ther.e.suitsshowedthattheimmuneeffectsofinactivatedoil—adjuvantvaccineandinaetivatedbacterinwerebetterthanthatofbait—absorbedvaccine.Outofalltreatments.injecting0.1mLinactivatedbactefinand0.3mLnornlalsalineinjectedt
6、oAeromonashydrophi上aand0.4mLinactivatedoil—adjuvantvaccinetoEdwardsieUawerefoundtobetllebestat85.0%and90.O%ofvaccineprotectiverate,respectively.neantibioticstherapytestingshowedthatflorfenicol,enrofloxacin,eiprofloxacinandnorfloxacinhadgoodtherapeuticeffectsontilapiainfectedwit
7、hAeromonashydrophilaandEdwardsiellafordecreasingtheincidencerateanddeathrate,andamongthem,20mg/kgH20florfenicolWaSfoundtobethebestincontrollingthesetwopathogens.Keywords:tilapia;Aemmonashydrophila;Edwardsiella;inactivatedvaccine;protectiveimmunity;medicinetherapy嗜水气单胞菌(Aeromona
8、shydrophila)隶属弧菌科(Vibrionaceae)、气单胞菌属(Aeromonas),可引起鱼类
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