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1、EffectofdifferentcarbonsourceandnitrogensourceonthecoldresistanceofcitruscallusinductionHuSiyuan(CollegeofAgronomy,NorthwestA&FUniversity,Yangling,Shaanxi712100,China)Abstract:Citruscallusisidealmaterialforphysicalandchemicalmutagenesisandgenetictransfor
2、mation,thecarbonsourceandnitrogensourceisanecessarycomponentintheinductionandsubcultureofcitruscallus,sotheexplorationofcarbon,nitrogensourcesisofgreatsignificancetotheeffectdegreeofcitruscallusinductionandeffectway.Thisstudychoosecoldresistanceofcitrusa
3、stestmaterial,throughculturevitrotissues,analysisoftheeffectsofcarbonsourceandconcentration,nitrogensourceandlevelontheinfluenceofcitruscallusinduction,ToprovidethebasisforthestudyoftheoccurrenceandthemechanismofcoldresistanceofCitrusgeneticimprovement,s
4、omaticembryos.Keywords:carbonsource;nitrogensource;citrus;callusIntroductionCitrus(CitrusreticulataBlanco)RutaceaeCitrus,smallevergreentreeorshrub,aboutthreemetershigh,weakbranches,usuallywiththorns.Leavesarealternate,leathery,lanceolatetoovate-lanceolat
5、e,5.5-8cmlong,2.9-4cmwide,apexacuminate,basecuneate,entireorwithfinebluntteeth,petioleslender,wingedobvious.Flowerssmall,yellow-white,solitaryorclusteredinleafaxils,sepals5;petals5;stamens18-24,filaments3-5piecesoftenconnate,ovary9-15rooms.Citrusfruitfla
6、tspherical,5-7cmindiameter,orange-yelloworreddishyellow,peelloosefleshmeateasilyseparated.Chenggucitruscultivationhasalonghistorydatingbackmorethan2,000yearsofhistory.ChengguCountyislocatedinSouthernShaanxi,CentralHanzhongBasin,northsubtropicaltotemperat
7、etransitionregion,northofQinlingmountainblockingtheinvasionofcoldnorthwest,southwindsBashansowarmaircannotdrivestraight.InversioneffectQinlingbeltandfoothillsslopingwall,formingauniquemicro-climateregions,fewerwintercoldandsummerheatless.First,here'scitr
8、ushightemperaturehotwinddoesnotharmflowering,fruitsettingrate;Secondcoolearlyautumn,temperature,andisconducivetofruitcolor,ripe,citrusfruitsurfacehereisgorgeous,highsolublesolidscontent,moderatelysweetandsour.Studies[1-4]h
