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1、....EffectofdifferentcarbonsourceandnitrogensourceonthecoldresistanceofcitruscallusinductionHuSiyuan(CollegeofAgronomy,NorthwestA&FUniversity,Yangling,Shaanxi712100,China)Abstract:Citruscallusisidealmaterialforphysicalandchemicalmutagenesisandgenetictransformation,t
2、hecarbonsourceandnitrogensourceisanecessarycomponentintheinductionandsubcultureofcitruscallus,sotheexplorationofcarbon,nitrogensourcesisofgreatsignificancetotheeffectdegreeofcitruscallusinductionandeffectway.Thisstudychoosecoldresistanceofcitrusastestmaterial,throug
3、hculturevitrotissues,analysisoftheeffectsofcarbonsourceandconcentration,nitrogensourceandlevelontheinfluenceofcitruscallusinduction,ToprovidethebasisforthestudyoftheoccurrenceandthemechanismofcoldresistanceofCitrusgeneticimprovement,somaticembryos.Keywords:carbonsou
4、rce;nitrogensource;citrus;callusIntroductionCitrus(CitrusreticulataBlanco)RutaceaeCitrus,smallevergreentreeorshrub,aboutthreemetershigh,weakbranches,usuallywiththorns.Leavesarealternate,leathery,lanceolatetoovate-lanceolate,5.5-8cmlong,2.9-4cmwide,apexacuminate,base
5、cuneate,entireorwithfinebluntteeth,petioleslender,wingedobvious.Flowerssmall,yellow-white,solitaryorclusteredinleafaxils,sepals5;petals5;stamens18-24,filaments3-5piecesoftenconnate,ovary9-15rooms.Citrusfruitflatspherical,5-7cmindiameter,orange-yelloworreddishyellow,
6、peelloosefleshmeateasilyseparated.Chenggucitruscultivationhasalonghistorydatingbackmorethan2,000yearsofhistory.ChengguCountyislocatedinSouthernShaanxi,CentralHanzhongBasin,northsubtropicaltotemperatetransitionregion,northofQinlingmountainblockingtheinvasionofcoldnor
7、thwest,southwindsBashansowarmaircannotdrivestraight.InversioneffectQinlingbeltandfoothillsslopingwall,formingauniquemicro-climateregions,fewerwintercoldandsummerheatless.First,here'scitrushightemperaturehotwinddoesnotharmflowering,fruitsettingrate;Secondcool学习参考....
8、earlyautumn,temperature,andisconducivetofruitcolor,ripe,citrusfruitsurfacehereisgorgeous,highsolublesolidscontent,moderatelysweetandsour.S
