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1、J.gen.Virol.(1986),67,2825-2831.PrintedinGreatBritain2825Keywords:IBV/spikeprecursor/sequencecomparisonComparisonoftheSpikePrecursorSequencesofCoronavirusIBVStrainsM41and6/82withthatofIBVBeaudetteByMATTHEWM.BINNS,*MICHAELE.G.BOURSNELL,FIONAM.TOMLEYANDT.DAVIDK.BROWNHoughtonPoultry
2、ResearchStation,Houghton,Huntingdon,CambridgeshirePE172DA,U.K.(Accepted29August1986)SUMMARYThenucleotidesequencesofthespikeprecursorgenesofinfectiousbronchitisvirusstrainsM41and6/82havebeendeterminedandcomparedwiththatoftheBeaudettestrainwhichwehavepreviouslysequenced.ThetwoMassa
3、chusettsstrains,M41andBeaudette,werefoundtoberemarkablysimilar,havingonly3.7~ooftheaminoacidsdifferent.Thesituationwith6/82,oneofthenewfieldisolates,isquitedifferentandthisstrainhad13.8~ofitsaminoacidsdifferentfromBeaudette.Thedifferencesidentifiedarediscussedintermsofthestructur
4、alfeaturesofthespikeprotein.Thecomparisonoftheaminoacidsequencesofviralproteinsfromdifferentviralstrainsandserotypeshasyieldedmuchusefulinformation.Thisapproachhasbeenusedtohelplocateconserveddomainsinproteinswhichmightbeessentialfortheirstructureandfunction,toidentifyepitopesinv
5、olvedinvirusneutralization(Minoretal.,1983),andtostudyantigenicdrift(Gethingetal,,1980).Infectiousbronchitisvirus(IBV),acoronavirus,causesaneconomicallyimportantdiseaseofpoultry.Therearealargenumberofserotypesofthevirus.Recently,anumberofstrainshavebeenisolatedwhicharenotrelateds
6、erologicallyinneutralizationteststostrainsofIBVpreviouslyisolatedinEngland(Cook,1984).ThepurposeofthepresentstudywastoseehowcloselyrepresentativestrainsofIBVarerelatedatthelevelofthespikeprotein,thevirusstructureagainstwhichneutralizingantibodiesaredirected(Mockettetal.,1984).Weh
7、averecentlyreportedthenucleotidesequenceofthegeneencodingthespikeprecursorproteinofIBVstrainBeaudette(Binnsetal.,1985a).TheBeaudettestrain,whichisnolongerpathogenicforchickens,isaMassachusetts-derivedstrainwhichhasbeenpassagedover250timesineggs.Inthispaperwepresentthespikesequenc
8、esoftheIBVstrainsM41and6/82,andcompareth
