冠状病毒Coronavirusl论文-1986 Coronavirus IBV_ Partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-.pdf

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1、VirusResearch,4(1986)133-143133ElsevierVRR00231CoronavirusIBV:partialaminoterminalsequencingofspikepolypeptideS2identifiesthesequenceArg-Arg-Phe-Arg-ArgatthecleavagesiteofthespikeprecursorpropolypeptideofIBVstrainsBeaudetteandM41DavidCavanagh,PhilipJ.DavisI.DarrylJ.C.Pappin2,MatthewM.Binnsi,Michae

2、lE.G.BoursnellandT.DavidK.BrownHoughtonPoultryResearchStation,Houghton,Huntingdon.Cumhs.PEI7_DA,andDepartmentofBlochrmrstry,lJnir>ersityofLeeds,LeedsLS-7UJT,U.K.(Acceptedforpublication4October1985)SummaryThespikeproteinofavianinfectiousbronchitiscoronaviruscomprisestwoglycopolypeptidesSlandS2deriv

3、edbycleavageofaproglycopolypeptideSo.thenucleotidesequenceofwhichhasrecentlybeendeterminedfortheBeaudettestrain(Binns,M.M.etal.,1985,J.Gen.Virol.66,719-726).TheorderofthetwoglycopolypeptideswithinSoisaminoterminus(Sl-S2-carboxyterminus(C).TolocatetheN-terminusofS2wehaveperformedpartialaminoacidseq

4、uencingonS2fromIBV-Beaudettelabelledwith[HlserineandfromtherelatedstrainIBV-M41labelledwith[3H]valine,leucineandisoleucine.TheresiduesidentifiedandtheirpositionsrelativetotheN-terminusofS2were:serine.13;valine,6,12;leucine.noneinthefirst20residues:isoleucine,2,19.TheseresultsidentifiedtheN-terminu

5、sofS2ofIBV-Beaudetteasserine.520residuesfromtheN-terminusofSl.excludingthesignalsequence.ImmediatelytotheN-terminalsideofresidue520SohasthesequenceArg-Arg-Phe-Arg-Arg;similarbasicconnectingpeptidesareafeatureofseveralothervirusspikeglycoproteins.ItwasdeducedthatforIBV-BeaudetteSlcomprises519residu

6、es(M,57.OK)or514residues(56.2K)iftheconnectingpeptidewastoberemovedbycarboxypeptidase-likeactivityinvivowhileS2has625residues(69.2K).NucleotidesequencingofthecleavageregionoftheSogeneofIBV-M41revealedthesameconnectingpeptideasIBV-Beaudetteandthatthefirst20N-terminalresiduesofS2ofIBV-M41wereidentic

7、altothoseoftheBeaudettestrain.IBV-BeaudettegrowninVerocellshadsomeuncleavedSo;thiswascleavableby10pg/mloftrypsinandofchymotrypsin.0168-1702/86/$03.50Q1986ElsevierSciencePuhhshersB.V.(BiomedicalDivision)134Partial