英语国际会议PPT.ppt

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1、1MacrophagesfrompatientswithatopicdermatitisshowareducedCXCL10expressioninresponsetostaphylococcala-toxin.CONTENTSIntroductionResultsMaterialsandMethods2Discussion3IntroductionkeywordspresentstudypurposebackgroundKeywordsatopicdermatitis;(特应性皮炎)chemokines;（

2、趋化因子）humanmacrophages;（人巨噬细胞）IP-10/CXCL10;MDC/CCL22;psoriasis;（牛皮癣；银屑病）Staphylococcusaureus;（金黄色葡萄球菌）α-toxin.（α-毒素）4Background:Patientswithatopicdermatitis(AD)andpsoriasisarefrequentlycolonizedwithStaphylococcusaureus(S.aureus).one-thirdofthemproducingα-tox

3、in,whichiscor-relatedwiththeseverityofeczemainAD.Distinctexpressionofchemokine(C-Cmotif)ligand(配体CCL)andchemokine(C-X-Cmotif)ligand(配体CXCL)chemokineshasbeendocumentedinbothdiseases.theeffectsofa-toxinonhumanmacrophagesinpatientswithADandpsoriasis?5Presentst

4、udy6Recentstudiesdemonstratedthatinfiltrationofinflammatorycellsintotissuesisregulatedbychemokines.Twomainsubfamilies,recentlyrenamedchemokine(C-Cmotif)ligand(CCL)andchemokine(C-X-Cmotif)ligand(CXCL)chemo-kines,havebeendistinguished.Severalstudieshavesugges

5、tedacrucialinvolvementofCCLchemokinesininflammation.ChemokinesandtheirreceptorsareimplicatedinthedevelopmentofsymptomsofADandpsoriasis.CXCL10playsaroleinpathogenesisofpsoriasis.Purpose7Theeffectsofα-toxinonhumanmacrophagesstillremainunclear.Weshowhereforthe

6、firsttimethatα-toxininducestheTh1-relatedchemokineCXCL10inhumanmacrophages.westudiedtheeffectsofα-toxinonTh1-andTh2-relatedchemokinesinmacrophagesfrompatientswithADandpsoriasiswheretheintrinsicabnormalanddifferentchemokinesproductionprofileiswelldefined.Mat

7、erialsandMethods8MaterialsandMethodsPatientsCellisolationandcultureStatisticalanalysisCytokineassessmentbyELISAWesternblotMethods9macrophage-derivedchemokine(MDC)/CCL22Cellsurfacemarkers’expressionandchemotaxisIFN-c-inducedproteinof10-kDa(IP-10)/CXCL10qRT–P

8、CRorELISAflowcytometryBoydenchambertechnique10PatientshealthydonorsandpatientswithADorpsoriasisCellisolationandculturePeripheralbloodmononuclearcells(PBMCs)wereisolatedbyLymphoprepdensity-gradientcentr