《英文文献演讲》PPT课件.ppt

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1、AberrantNeuregulin1SignalinginAmyotrophicLateralSclerosisLuKeClassc4178112177OUTLINEIntroductionMaterialsandMethodsResultsDiscussionIntroductionAmyotrophiclateralsclerosis(ALS肌萎缩性脊髓侧索硬化症)isaprogressiveneurodegenerativediseasethataffectsnervecellsinthebrainandthespinalco

2、rd.ThepathogenesisofALSispoorlyunderstood,andtherearepresentlynoeffectivetherapiestostopitsinsidiousprogression.IntroductionNeuregulin1(NRG1)isaneuron-derivedtrophicmoleculethatsupportsaxoglialandneuromusculardevelopmentthroughseveralalternativelysplicedisoformsItspossi

3、bleroleinthepathogenesisandprogressionofamyotrophiclateralsclerosis(ALS)isnotknown.CanNRG1beanoveltherapeutictargetagainstdiseaseprogressioninALS?MATERIALSANDMETHODSHumanTissueFresh-frozenthoracicandlumbarspinalcordsfrom6sporadicand2familialALSsubjectsand6controlpatient

4、s.SOD1MouseTissue（过氧化物歧化酶1转基因小鼠）Thoracicandlumbarspinalcordregionswerecollectedfromeachmouseatdifferentstages(preclinical/diseaseonset/end-stagedisease).4to8micewereusedpergroupateachtimepoint(4timepoints,32miceintotal).MATERIALSANDMETHODSHistopathologyImmunohistochemis

5、tryImmunoblottingQuantitativeImageAnalysisIsolationandReal-timeQuantitativeReverseTranscription-PolymeraseChainReactionStatisticalAnalysisRESULTSNRG1ReceptorActivationonMicrogliaDuringSOD1DiseaseProgressionandinALSDISCUSSIONNRG1-InducedMicroglialActivationasaPossibleThe

6、rapeuticTargetforALSDifferentRolesforAlternativelySplicedIsoformsofNRG1DifferencesandSimilaritiesinALSandtheSOD1MouseModelQ&ATHANKYOU