常用酿酒酵母菌株基因型.docx

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1、CommonlyusedstrainsThispagedescribessomeofthemostcommonlyusedyeastlabstrains.Muchoftheinformationistakenfrom F.Sherman(2002) Gettingstartedwithyeast,MethodsEnzymol.350,3-41.Otherusefulpapersforstrainbackgroundinformationinclude:§MortimerandJohnston (1986)Genetics113:35-43-th

2、oroughlydescribesthegenealogyofstrainS288C§vanDijkenetal. (2000)EnzymeMicrobTechnol26:706-714-comparesvariouscharacteristicsofcommonlyusedlabstrains§Winzeleretal. (2003)Genetics163:79-89-usesSFP(single-featurepolymorphisms)analysistostudygeneticidentitybetweencommonlabstrain

3、sS288CGenotype: MATα SUC2gal2malmelflo1flo8-1hap1hobio1bio6Notes: Strainusedinthesystematicsequencingproject,thesequencestoredinSGD.S288Cdoesnotformpseudohyphae.Inaddition,sinceithasamutatedcopyof HAP1,itisnotagoodstrainformitochondrialstudies.Ithasanallelicvariantof MIP1 wh

4、ichincreasespetitefrequency.S288Cstrainsare gal2-andtheydonotusegalactoseanaerobically.TheS288Cgenomewasrecentlyresequencedatthe SangerInstitute.References: MortimerandJohnston (1986)Genetics113:35-43.Sources: ATCC:204508BY4743Genotype: MATa/α his3Δ1/his3Δ1leu2Δ0/leu2Δ0LYS2/

5、lys2Δ0met15Δ0/MET15ura3Δ0/ura3Δ0Notes: Strainusedinthe systematicdeletionproject,generatedfromacrossbetweenBY4741andBY4742,whicharederivedfromS288C.AsS288c,thesestrainshaveanallelicvariantof MIP1 whichincreasespetitefrequency.SeeBrachmannetal.referencefordetails.References: 

6、Brachmannetal. (1998)Yeast14:115-32.Sources: Biosystems:YSC1050FY4Genotype: MATaNotes: DerivedfromS288C.References: Winstonetal. (1995)Yeast11:53-55.Brachmannetal. (1998)Yeast14:115-32.FY1679Genotype: MATa/α ura3-52/ura3-52trp1Δ63/TRP1leu2Δ1/LEU2his3Δ200/HIS3GAL2/GALNotes: I

7、sogenictoS288C;usedinthesystematicsequencingproject,thesequencestoredinSGD.References: Winstonetal. (1995)Yeast11:53-55.Sources: EUROSCARF:10000DAB972Genotype: MATα X2180-1Btrp10 [rho 0]Notes: IsogenictoS288C;usedinthesystematicsequencingproject,thesequencestoredinSGD.AB972i

8、sanethidiumbromide-inducedrho-derivativeofthestrainX2180-1B-trp1.References