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1、SimultaneousDistillationExtractionofSomeVolatileFlavorComponentsfromPu-erhTeaSamples—ComparisonwithSteamDistillation-Liquid/LiquidExtractionandSoxhletExtractionXungangGu,ZhengzhuZhang,XiaochunWan,JingmingNing,ChengchengYao,andWanfangShaoKeyLabofTeaBiochemistry&Biotechnology,Ministryof
2、Agriculture,AnhuiAgriculturalUniversity,Hefei230036,China2InstituteofPu-erhTea,YunnanAgriculturalUniversity,Kunming650201,China三·结果与讨论1.色谱表征：(1)GC毛细管柱DB-5柱（极性柱30m×0.25mm,薄片厚度0.25μm）(2)升温程序如上实验部分(3)GC-FID和GC-MS的标准物谱图和普洱茶生茶的色谱图图1.标准化合物色谱图1=Benzylalcohol;2,3=Linalooloxide;4=Linalool;5=Ph
3、enethylalcohol;6=α-Terpineol;7=Geraniol;8=1,2,3Trimethoxylbenzene;9=1,2,4Trimethoxylbenzene;IS=internalstandard;10=Nerolidol.(a)GC-FID谱图(b)GC-MS谱图图2.普洱茶生茶样品谱图(a)Pu-erhrawteaanalyzedbyGC-FID(b)Pu-erhrawteaanalyzedbyGC-MS1=Benzylalcohol;2,3=Linalooloxide;4=Linalool;5=Phenethylalcohol;6=
4、α-Terpineol;7=Geraniol;8=1,2,3Trimethoxylbenzene;9=1,2,4Trimethoxylbenzene;IS=internalstandard;10=Nerolidol.Table1:标准化合物色谱参数Peakno.CompoundRetentiontime(min)Concentration(μg·mL−1)PeakareaRelativefactor∗1Benzylalcohol苯甲醇10.2326.88194.11.09512and3Linalooloxide芳樟醇氧化物11.59+12.1570.61275.1
5、2.02964Linalool芳樟醇12.5355.18153.12.855Phenethyalcohol苯乙醇13.0728.24173.11.29016α-Terpineolα-松香醇15.9227.02217.60.98847Geraniol香叶醇18.2199626.51.249681,2,3-Trimethoxylbenzene1,2,3-三甲氧基苯20.3246.57233.81.575191,2,4-Trimethoxylbenzene1,2,4-三甲氧基苯22.3548.22271.81.4029ISIS（内标物）癸酸乙酯23.117.3136.8
6、110Nerolidol橙香叔醇28.6552.56439.70.9452∗Relativefactor:f=(Ci×Ais)/(Ai×Cis)3.9554.4214.676Nerolidol橙香叔醇5.7464.2943.1741,2,4-Trimethoxylbenzene1,2,4-三甲氧基苯11.844.30513.261,2,3-Trimethoxylbenzene1,2,3-三甲氧基苯4.06130.7270.35Geraniol香叶醇51.6225.6359.04α-Terpineolα-松香醇10.5417.1918.83Phenethyalcoh
7、ol苯乙醇36.62280.5658.1Linalool芳樟醇23.9633.6961.05Linalooloxide芳樟醇氧化物15.116.0620.13Benzylalcohol苯甲醇SoxhletSD-LLESDECompoundname∗Relativefactor:f=(Ci×Ais)/(Ai×Cis)2·三种提取方法的比较(组分单位：μg·mL−1)SDE适合高挥发性成分索氏提取适合高沸点（低挥发性）化合物CompoundnameSDESpikedlevelMeanRecoveryRSD(μg·mL−1）(μg·mL−1）(μg·mL−1）(%,n=
8、3)(%,
