冠状病毒Coronavirusl论文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ Genotype-Specific Detection of Ferret Coronavirus by Convent.pdf

冠状病毒Coronavirusl论文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ Genotype-Specific Detection of Ferret Coronavirus by Convent.pdf

ID:50678574

大小:460.87 KB

页数:18页

时间:2020-03-16

冠状病毒Coronavirusl论文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ Genotype-Specific Detection of Ferret Coronavirus by Convent.pdf_第1页
冠状病毒Coronavirusl论文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ Genotype-Specific Detection of Ferret Coronavirus by Convent.pdf_第2页
冠状病毒Coronavirusl论文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ Genotype-Specific Detection of Ferret Coronavirus by Convent.pdf_第3页
冠状病毒Coronavirusl论文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ Genotype-Specific Detection of Ferret Coronavirus by Convent.pdf_第4页
冠状病毒Coronavirusl论文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ Genotype-Specific Detection of Ferret Coronavirus by Convent.pdf_第5页
资源描述:

《冠状病毒Coronavirusl论文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ Genotype-Specific Detection of Ferret Coronavirus by Convent.pdf》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库

1、Chapter16Genotype-SpecificDetectionofFerretCoronavirusbyConventionalandReal-TimeReverseTranscriptionPolymeraseChainReactionAnnabelG.Wise,MattiKiupel,andRogerK.MaesAbstractFerretcoronavirusisassociatedwithtwodiseasepresentationsinferrets,namely,epizooticcatarrhalenteri-tisandafelineinfectiousperiton

2、itis(FIP)-likesystemicdisease.Inthischapter,wedescribeconventionalandreal-timeone-stepreversetranscriptionpolymerasechainreactionassaysthatareroutinelyusedinourlaboratorytodetecteithergenotype1orgenotype2ferretcoronavirusinclinicalspecimens.Theseassaysweredesignedbasedupontheconservedspikegenesequ

3、encedifferencefoundbetweenthreestrainsofferretsystemiccoronavirusandthreestrainsofferretentericcoronavirus.Recentliteratureevidenceindi-catesthatpathotypeisnotassociatedwithaspeciÞcgenotype,andtherefore,itisimportanttotestforbothgenotypeseitherinentericorsystemicdisease.KeywordsFerretcoronavirus,C

4、onventionalRT-PCR,Real-timeRT-PCR,Moleculardiagnosticassay1IntroductionEpizooticcatarrhalenteritis(ECE),anentericdiseaseofdomesticferretswasÞrstdescribedintheUSAin1993[1].Clinically,thediseaseischaracterizedbyafoul-smellingbrightgreendiarrheawithhighmucuscontent,lethargy,anorexia,andvomiting.Morbi

5、dityapproaches100%,butmortalityisusuallybelow5%,withjuvenileferretsoftendevelopingonlymilddisease.ECEwasfoundtobeassociatedwithanalphacoronavirus,designatedferretentericcoronavirus(FRECV)[2].Thisdiseaseortheentericcoro-navirusagentassociatedwithithassincespreadintheUSAandothercountriesworldwide[3Ð

6、5].Closetoadecadeaftertherec-ognitionofECE,asystemicdisease,characterizedbyfelineinfec-tiousperitonitis(FIP)-likeclinicalsignsandlesions,begantoemergeinferretsacrosstheUSAandEurope[6Ð9].ClinicalÞnd-ingsincludeanorexia,weightloss,diarrheaandthepresenceofLeyiWang(ed.),AnimalCoronaviruses,SpringerPro

7、tocolsHandbooks,DOI10.1007/978-1-4939-3414-0_16,©SpringerScience+BusinessMediaNewYork2016171172AnnabelG.Wiseetal.largepalpableintra-abdominalmasses.AmarkedresemblancetoFIPisthegrossobservationofwidespreadwhitishn

当前文档最多预览五页,下载文档查看全文

此文档下载收益归作者所有

当前文档最多预览五页,下载文档查看全文
温馨提示:
1. 部分包含数学公式或PPT动画的文件,查看预览时可能会显示错乱或异常,文件下载后无此问题,请放心下载。
2. 本文档由用户上传,版权归属用户,天天文库负责整理代发布。如果您对本文档版权有争议请及时联系客服。
3. 下载前请仔细阅读文档内容,确认文档内容符合您的需求后进行下载,若出现内容与标题不符可向本站投诉处理。
4. 下载文档时可能由于网络波动等原因无法下载或下载错误,付费完成后未能成功下载的用户请联系客服处理。