冠状病毒Coronavirusl论文-2012 On Channel Activity of Synthetic Peptides Derived from Severe and Acute Respiratory Syndrome Coronavirus (SARS-CoV).pdf

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1、656aWednesday,February29,2012ethanolamine(PE18:1/16:0)andphosphatidylserine(PS18:0/18:1).Sincethis3332-PosBoardB193membraneisthickerthantheestimatedlengthoftolaasinchannel,mismatchinElectricalAspectsofMembranePermeabilizationbyNewPolycationicthicknessmaymakethechannelunstable.Ph

2、ospholipidscomposedofmediumPeptidesDerivedfromtheCry11BbProtoxinorshort-chainfattyacidsmaybehelpfultothestabilityoftolaasinchannelbyVictorV.Lemeshko,JoseA.Alvarez.makingthemembranethinner.WhenphosphatidylethanolaminesmadewithNationalUniversityofColombia,Medellin,Colombia.decanoi

3、cacids(capricacid,DDPE),myristicacids(DMPE),andstearicacidsThepermeabilizationofmitochondrialandplasmamembranesbysynthetic(DSPE)wereadded,DDPE(200nM)facilitatedtolaasin-inducedhemolysis.polycationicpeptidesderivedfromtheCry11Bbprotoxinwasstudied.TheWhentheconcentrationofDDPEwasa

4、djustedfrom0.2-200nM,thehemoly-peptidesweredesignedwiththeaimtofurtherstudyoftheirantimicrobialsiswasstimulatedattheconcentrationsabove2nM.KsvalueofDDPEeffectandanticanceractivities.Itwasobservedthatthemembranepermeabilizingwasobtainedat6mMDDPE.Whenthepreincubationeffectoftolaas

5、inandactivityofthesepolycationicpeptidesstronglyincreasedbythetransmem-DDPEwasmeasured,bindingoftolaasintoDDPEwascompletedwithin5branepotential(minusinside).Thisphenomenonwasconfirmedbythestudymin.Inthelipidbilayerrecording,theadditionofDDPEincreasedtolaasinoftheartificialplanarme

6、mbranepermeabilization:applying50mVtothepla-channelactivitybyincreasingopenprobability.Therefore,tolaasinmoleculesnarmembrane(minustothetransside)during30secinducedtime-dependentmakemorestablechannelswithphospholipidscomposedofmedium-chainincreaseinthetransmembranecurrentinthepr

7、esenceofapeptideaddedtofattyacids.thecisside,whilesubsequentapplicationoftheoppositepotentialcauseditsdecrease.Wealsoobservedthattheactivationofthecellsuicidemecha-3330-PosBoardB191nism,whichpartiallyrevealedinphosphatidylserineexposureatthecellsur-2DCompetitionEffectofDDPEandZn

8、ontheHemolysisInducedbyface,significantlyincreas