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1、287CurrentTopicsinMicrobiologyandImmunologyEditorsR.W.Compans,Atlanta/GeorgiaM.D.Cooper,Birmingham/AlabamaT.Honjo,Kyoto·H.Koprowski,Philadelphia/PennsylvaniaF.Melchers,Basel·M.B.A.Oldstone,LaJolla/CaliforniaS.Olsnes,Oslo·M.Potter,Bethesda/MarylandP.K.Vogt,LaJolla/California·H.Wagner,MunichL.Enjua
2、nes(Ed.)CoronavirusReplicationandReverseGeneticsWith49FiguresProfessorDr.LuisEnjuanesCentroNacionaldeBiotecnologíaDepartmentofMolecularandCellBiologyCampusUniversidadAutónoma,Cantoblanco38049MadridSpaine-mail:L.Enjuanes@cnb.uam.esCoverillustrationbyLuisEnjuanesSimplifiedstructureofacoronaviruspro
3、totype(transmissiblegastroenteritisvirus,TGEV)showingtheenvelopewithseveralstructuralproteins:spike(S),twotopologiesofthemembrane(MorM')proteins,envelope(E)protein,andnucleoprotein(N).Anucleocapsidcanbeobservedinsidetheenvelopevirus,containingthegenomicRNA,theNprotein,andthecarboxiterminusoftheMp
4、rotein.LibraryofCongressCatalogCardNumber72-152360ISSN0070-217XISBN3-540-21494-1SpringerBerlinHeidelbergNewYorkThisworkissubjecttocopyright.Allrightsarereserved,whetherthewholeorpartofthematerialisconcerned,specificallytherightsoftranslation,reprinting,reuseofillustrations,recitation,broadcasting
5、,reproductiononmicrofilmsorinanyotherway,andstorageindatabanks.DuplicationofthispublicationorpartsthereofispermittedonlyundertheprovisionsoftheGermanCopyrightLawofSeptember9,1965,initscurrentversion,andpermissionforusemustalwaysbeobtainedfromSpringer-Verlag.Violationsareliableforprosecutionundert
6、heGermanCopyrightLaw.SpringerisapartofSpringerScience+BusinessMediaspringeronline.comSpringer-VerlagBerlinHeidelberg2005PrintedinGermanyTheuseofgeneraldescriptivenames,registerednames,trademarks,etc.inthispublicationdoesnotimply,evenintheabsenceofaspecificstatement,thatsuchnamesareexemptfromther
7、elevantprotectivelawsandregulationsandthereforefreeforgeneraluse.Productliability:Thepublisherscannotquaranteethataccuracyofanyinformationaboutdosageandapplicationcontainedinthisbook.Ineveryindividualcasetheusermustche
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