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1、I&wResearch,22(1992)125-14112501992ElsevierSciencePublishersB.V.Allrightsreserved0168-1702/92/$05.00VIRUS00728Sequenceanalysisofhumancoronavirus229EmRNAs4and5:evidenceforpolymorphismandhomologywithmyelinbasicproteinPatriciaJouvenner,SamirMounir,JanetN.Stewart,ChristopherD.Richardson*a
2、ndPierreJ.TalbotIInstitutArmand-Frappier,Universite’duQw’bec,virologyResearchCenter,Laval,Q&bee,Canadaand2BiotechnologyResearchCenter,NationalResearchCouncilofCanada,Mont&al,Quibec,Canada(Received1May1991;revisionreceivedandaccepted31October1991)SummaryHumancoronaviruses(HCV)areimport
3、antpathogensresponsibleforrespira-tory,gastrointestinalandpossiblyneurologicaldisorders.TobetterunderstandthemolecularbiologyoftheprototypeHCV-229Estrain,thenucleotidesequenceofthe5-uniqueregionsofmRNAs4and5weredeterminedfromclonedcDNAs.SequenceanalysisofthecDNAssynthesizedfrommRNA4re
4、vealedamajordifferencewithpreviouslypublishedresults.However,polymerasechainreactionamplificationofthisregionshowedthatthesequencedcDNAswereproducedfromminorRNAspecies,anindicationofpossiblegeneticpolymorphisminthisregionoftheviralgenome.ThemutatedmessengerRNA4containstwoORFs:(1)ORF4a
5、consistingof132nucleotideswhichpotentiallyencodesa44-aminoacidpolypeptideof4653Da;thiscodingsequenceisprecededbyaconsensustranscrip-tionalinitiationsequence,CUAAACU,similartotheonesfoundupstreamoftheNandMgenes;(2)ORF4bof249nucleotidespotentiallyencodingan83-aminoacidbasicandleucine-ri
6、chpolypeptideof9550Da.Ontheotherhand,mRNA5containsonesingleORFof231nucleotideswhichcouldencodea77-aminoacidbasicandleucine-richpolypeptideof9046Da.Thisputativeproteinpresentsasignificantdegreeofaminoacidhomology(33%)withitscounterpartfoundintransmissiblegastroenteritiscoronavirus(TGEV
7、).Theproteinsinthetwodiffer-entvirusesexhibitsimilarmolecularweightsandareextremelyhydrophobic.Correspondenceto:P.J.Talbot,CentredeRechercheenVirologie,InstitutArmand-Frappier,531boul.desPrairies,Lava],Qui.,CanadaH7N423.126Interestingly,asequencehomologyoffiveaminoacidswasfoundbetween
8、thepr
