冠状病毒Coronavirusl论文-1993 An immunodominant CD4+ T cell site on the nucleocapsid protein of murine coronavirus contributes to protection agai.pdf

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1、JournalofGeneralVirology(1993),74,1287-1294.PrintedinGreatBritain1287AnimmunodominantCD4÷TcellsiteonthenucleocapsidproteinofmurinecoronaviruscontributestoprotectionagainstencephalomyelitisHelmutWege,*AndreasSchliephake,HeinerK6rner,~EgbertFloryandHannaWegeInstituteofVirologyandImmunobiology,Uni

2、versityofWiirzburg,VersbacherStrafle7,7078Wiirzburg,GermanyThemurinecoronavirusneurotropicstrainJHM(MHV-proteinfragments.Thecarboxy-terminallylocatedC4-NJHM)nucleocapsidCN)proteininducesastrongT-fragment(95aminoacids)inducedthemostpronouncedhelpercellresponseinLewisrats.Ithasbeenshownproliferat

3、iveresponseirrespectiveofwhetherthelym-previouslythatN-specificCD4÷TcellscanconferphocyteculturewasderivedfromimmunizedorMHV-protectionagainstacutediseaseupontransfertoJHM-infectedrats.WeestablishedTcelllinesspecificotherwiselethallyinfectedrats.TodefinethemajorforthetruncatedNproteinfragmentsa

4、ndtestedtheirantigenicregionsthatelicitthisTcellresponse,potentialtomediateprotectionbytransferexperiments.truncatedfragmentsofNproteinwereexpressedfromaOnlytheTcelllineC4-NandtheTcelllinespecificforbacterialexpressionvectorandemployedasTcellthefull-lengthNproteinwereprotective.Bycontrast,allan

5、tigens.LymphocytesfromeitherMHV-JHM-infectedtruncatedNproteinfragmentselicitedahumoralorimmunizedratswerestimulatedinculturewithvirusimmuneresponseandcontainedantigenicsitesrecog-antigen,grownandtestedfortheirspecificitytotheNnizedbyantibodiesfromdiseasedrats.Introductionstructureandfunctionofv

6、iralproteinsandtheirinteractionswiththeimmunesystemduringthecourseofCoronavirusesareresponsibleforavarietyofimportantinfection.diseasesintheirnaturalhosts(Wegeetal.,1982).TheStudiesoncoronavirusinfectionsrevealedthatthedifferentformsofencephalomyelitisinducedbymurineCD4÷Tcellresponseisalsoofpri

7、meimportanceinthiscoronaviruses(MHV)inrodentshavereceivedmuchsystem.However,therelativecontributionofcellularattentionasamodelforinvestigatingpossiblemechan-andhumoralimmunitydiffersaccordingtothevirus-ismsofvirus-induceddemyelina