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1、_AmhivesArchVirol(1993)130:279-288Vrology©Springer-Verlag1993PrintedinAustriaThegeneencodingthenucleocapsidprotein:sequenceanalysisinmurinehepatitisvirustype3andevolutioninCoronaviridaeD.D~cimo1'2,H.Philippe3,MichelleHadchouel1,M.Tardieu1'2,andM.Meunier-Rotival11INSERMUnit6347affiliSeauCNRSa
2、nd2LaboratoiredeNeurovirologieetNeuroimmunologie,Universit6ParisXI,H6pitaldeKremlin-BicStre3LaboratoiredeBiologieCellulaire4(URACNRS1134),Universit6ParisXI,Orsay,FranceAcceptedDecember15,1992Summary.Thenucleoprotein-encodinggene(N)ofmurinehepatitisvirustype3(MHV3),fromtheMillHiltstrain,wascl
3、onedandsequenced.ItwascomparedtogeneNfromotherroutinecoronavirusesandwasfoundtosharemoresimilaritieswithNsequencesfromMHV1andMHVJHMstrainsthanwiththepublishedMHV3NsequencewhichisalmostidenticaltoMHVA59.WesuggestthattheevolutionofsomeMHVNsequencesresultedfromadoublerecombinationphenomenonbetw
4、eentwoancestors.Furthermore,comparisonofproteinNfromavianandmammaliancoronavirusesleadstothehypothesisthathorizontaltransfereventsofthevirusfromonehostspeciestoanotherhaveoccurred.IntroductionMurinehepatitisviruses(MHV)areenvelopedRNAvirusesbelongingtothefamilyCoronaviridae.Theirgenomeisapol
5、yadenylated,non-segmented,singlestrandedRNA]-20]codingforthreetofourmajorstructuralproteinsaccordingtotheantigenicgroup:thespikeglycoprotein(S),theHEantigen,themembraneassociatedmatrixglycoprotein(M),andtheinternalnucleocapsidprotein(N),inadditiontonon-structuralproteinssuchasanRNA-dependant
6、RNApo-lymerase[18].Theorderofthegenesfrom5'to3'is:RNApolymerase,HE,S,M,N[9].Duringreplication,afull-lengthnegative-strandedRNAandsixpolyadenylatedmessengerRNAsaresynthesizedinadditiontothefulllengthpositive-senseRNA.ThesubgenomicRNAsformanestedset,alloverlappingwiththe3'endofthegenomicRNA[10
7、].AmongtheMHVgroup,differentstrainsinducevariouspathologies[27].280D.D6cimoetal.IntraperitonealinjectionofMHV3fromtheMillHillstrain(MHV3MH)inadultmiceresultsinfutminanthepatitisorinchronicinfectionofbrain[23,26].InordertobettercharacterizeMHV3MHand
