冠状病毒Coronavirusl论文-2007 Enhancement of Murine Coronavirus Replication by Severe Acute Respiratory Syndrome Coronavirus Protein 6 Requires t.pdf

冠状病毒Coronavirusl论文-2007 Enhancement of Murine Coronavirus Replication by Severe Acute Respiratory Syndrome Coronavirus Protein 6 Requires t.pdf

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时间:2020-03-16

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1、JOURNALOFVIROLOGY,Oct.2007,p.1152011525Vol.81,No.200022-538X/07/$08.000doi:10.1128/JVI.01308-07Copyright©2007,AmericanSocietyforMicrobiology.AllRightsReserved.NOTESEnhancementofMurineCoronavirusReplicationbySevereAcuteRespiratorySyndromeCoronavirusProtein6RequirestheN-TerminalHydrophobicRegi

2、onbutNotC-TerminalSortingMotifs1223JasonNetland,DebraFerraro,LeciaPewe,HeidiOlivares,31,2ThomasGallagher,andStanleyPerlman*12InterdisciplinaryPrograminImmunologyandDepartmentofMicrobiology,UniversityofIowa,IowaCity,Iowa,and3DepartmentofMicrobiologyandImmunology,LoyolaUniversityMedicalCenter,

3、Maywood,IllinoisReceived14June2007/Accepted23July2007Severeacuterespiratorysyndromecoronavirusencodesseveralaccessoryproteinsofunknownfunction.Wepreviouslyshowedthatonesuchprotein,encodedbyORF6,enhancedthegrowthofmousehepatitisvirusintissueculturecellsandinmice.Protein6consistsofanN-terminalh

4、ydrophobicpeptideandaC-terminalregioncontainingintracellularproteinsortingmotifs.Herein,weshowthatmutationofthehydrophobicregionbutnotthesortingmotifsaffectedtheabilityofprotein6toenhancevirusgrowth.Collectively,theseresultssupportthenotionthatthe6proteininteractswithmembrane-boundviralreplic

5、ationorassemblymachinerytodirectlyenhancevirusreplicationandvirulenceinanimals.Theetiologicalagentofthesevereacuterespiratorysyn-amphipathicportionortheputativeproteinsortingmotifsindrome(SARS)wasidentifiedasacoronavirus(SARS-CoV).theCterminusareessentialforprotein6toinfluenceCoVInadditionaltos

6、tructuralproteins,SARS-CoVencodeseightinfections.accessoryproteins,whosefunctionsarenotwelldefined(15).Asinourpreviousstudies,weusedJ2.2-v-1astheparentalPreviously,weintroducedeachoftheSARS-CoVaccessoryvirus(13).VariantsweregeneratedbyPCRusingprimersgenesintoanattenuatedstrainofmousehepatitisv

7、irusencodingthedesiredORF6mutations(primersequences(MHV),strainJHMJ2.2-v-1,(J2.2-v-1),byusingreversege-availableuponrequest).Inthefirstsetofmutants,YSELandnetics(14).J2.2-v-1causesamildencephalitisfollowedbyadiacidicsortingmotifslocatednearthe

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