资源描述：
《冠状病毒Coronavirusl论文-1979 Preliminary studies on the isolation of coronavirus 229E nucleocapsids.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、FEMSMicrobiologyLetters5(1979)101-105101©CopyrightFederationofEuropeanMicrobiologicalSocietiesPublishedbyElsevier/North-HollandBiomedicalPressPRELIMINARYSTUDIESONTHEISOLATIONOFCORONAVIRUS229ENUCLEOCAPSIDSE.O.CAUL,C.R.ASHLEY,MORAGFERGUSON*andS.I.EGGLESTONE**PublicHea
2、lthLaboratory,MyrtleRoad,Kingsdown,BristolBS28EL,*DepartmentofVirology,BristolRoyalInfirmary,BristolBS28HWand**SchoolofEnvironmentalScience,PlymouthPolytechnic,PlymouthPL48AA,EnglandReceived6October19781.Introduction2.MaterialandMethodsCoronavirusesarealargegroupofe
3、nveloped,lipid2.1.Viruscontaining,RNAviruseswhicharecharacterisedbytheirdistinctiveprojectionmorphology[1].ThereAlaboratory-adaptedstrainoftherespiratoryhavebeenextensivereportsdescribingthemorpho-coronavirus229EwaskindlysuppliedbyDr.P.G.logyofthevirionbothinthinsec
4、tionsofinfectedHiggins.cellsandbythenegativestainingtechnique[2].Althoughseveralmembersofthisgrouphavebeen2.2.Cellculturescharacterizedbiochemically[3-7]littleisknownaboutthemorphologyoftheinternalcomponent.Acontinuouslineofhumanlungcells(MRC-C)ApostolovandFlewett[8
5、]observed7-8nmthreadswereusedatpassagelevels22-28intheseinvestiga-inthinsectionsofpelletedpreparationsofaviantions.ThesecellswereobtainedfromDr.S.Reed.infectiousbronchitisvirusandKennedyandJohnson-Lussenburg[9]isolatedtheinternalcomponentof2.3.Preparationoflabelledv
6、irusthehumanrespiratorycoronavirus229EanddescribeditsmorphologyfromnegativelystainedConfluentmonolayersofMRC-Ccellswerepreparations.However,Macnaughtonetal.[10]infectedwith229Evirusandsimultaneouslylabelledrecentlydescribedthemorphologyfollowingnegativewith100/~Ci/m
7、lof[5-3H]uridine(spec.act.5.0Ci/stainingofribonucleoprotein(RNP)-likestructuresinmmol).(TheRadiochemicalCentre,Amersham).Thetheirpreparationsof229Eviruswhichwereclearlycultureswereincubatedat35°Candviruswashar-dissimilar,bothindiameterandappearancetothosevestedat36h
8、postinfectionwhenthecytopathicpreviouslyreported[9].Nucleocapsid-likestructures,effectwas90-100%complete.9nmindiameter,havebeenobservedint
