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1、J.gen.Virol.(1984),65,645~i49.PrintedinGreatBritain645Keywords:Bernevirus/polypeptides/inhibitorsBerneVirusIsNot'Coronavirus-fike'ByM.C.HORZINEK,*M.WEISS1ANDJ.EDERVEENInstituteofVirology,VeterinaryFaculty,StateUniversity,Utrecht,Yalelaan1,3508TDUtrecht,TheNetherlandsandXVirologyDepartment
2、,InstituteofBacteriology,VeterinaryFaculty,UniversityofBerne,Switzerland(Accepted21November1983)SUMMARYIninfectedembryonicmuleskincells,Bernevirusdirectsthesynthesisoftwomainpolypeptides(22K,20K);inaddition,virus-specificproteinswithapparentmolecularweightsof>200K,80Ktot20K,32Kand17Kwered
3、etectedafterradioimmuneprecipitation.ThereplicationofBerneviruswasreducedmorethan1000-foldbyactinomycinD,whenthedrug(0.1to1-0gg/ml)wasaddedduringthefirst8hafterinfection;alpha-amanitin(25~g/ml)producedasimilarthoughlesspronouncedeffect.U.v.preirradiationofthecellsfor>i5sledtoadramaticdecr
4、easeintheproductionofextracellularvirus.TheresultspresentedsupportoursuggestionthatBernevirusisarepresentativeofanewfamilyofanimalviruses.Recently,wereportedthepurificationandpartialcharacterizationofanewenvelopedRNAviruswhichhadbeenisolatedfromahorseinBerne,Switzerland.'Bernevirus'measur
5、es120to140nminitslargestdiameterandconsistsofapeplomer-bearingenvelopeandanelongatedcorewhichisbentintoanopentoruswithinthemembrane.Itscurvedshapeisapparentlymaintainedbythetightlyfittingenvelope;fromthin-sectionelectronmicroscopicimagesweconcludedthattheBernevirionhastheuniqueshapeofa(bi
6、concave)disc.Thecoreistubularinappearanceandshowsatransversestriationindicativeofahelicalnucleocapsidsymmetry.BerneviruspossessesanRNAgenome:itsgrowthisnotaffectedbyiododeoxyuridine(IUdR)underconditionswhereequineherpesvirusreplicationisinhibitedbymorethan3log10unitsofinfectivity(Weisseta
7、l.,1983).WehaveshownthatBernevirusisserologicallyunrelatedtoinfectiousbronchitis,mousehepatitis(MHV)andtransmissiblegastroenteritisviruseswhicharerepresentativesofthreeantigenicclustersofcoronaviruses(Weissetal.,1983).Itisconceivable,however,thatBerneviruscouldstill
