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1、J.gen.Virol.(1988),69,621629.PrintedinGreatBritain621Keywords:inJeetiousbronchitisvirus/nucleotidesequenee/eeolutionEvolutionofAvianCoronavirusIBV:SequenceoftheMatrixGlycoproteinGeneandIntergenicRegionofSeveralSerotypesByDAVIDCAVANAGH*ANDPHILIPJ.DAVISAFRCInst
2、ituteJbrAnimalDiseaseResearch,HoughtonLaboratory,Houghton,Huntingdon,CambridgeshirePE172DA,U.K.(Accepted12Not'ember1987)SUMMARYWehavesequenced200to240basesofthematrix(M)glycoproteingeneof23strainsofinfectiousbronchitisvirus(IBV)representingtheA(D207),B(D3896)
3、,C(D3128),D(D212),Massachusetts(Mass),UKI1andUK12serotypes.Thebasesexaminedcodefortheexternal,hydrophilicregionandthefirstmembrane-embeddedhydrophobicregionofM,bothregionscomprisingapproximately20aminoacids.AspredictedfromproteinMrstudiestheA/DandB/Cserotypes
4、hadtwoandonepotentialglycosylationsitesrespectively.Thisvariationappearedtoderivefromacombinationofbasesubstitutionsanddeletions/insertions.TheglycosylationsequenceAsn-Cys-Thrwashighlyconserved.Overall,theexposedpartofMexhibitedafourfoldgreaterextentofaminoac
5、idvariationthandidthemembrane-embeddedsequence.Thetranscription-associatedhomologyregionsequence(CUUAACAA)inthe5'intergenicregionwasidenticalinallstrainsbuttherewasconsiderablevariationastoitslocation.TheMgeneofUK12appearedtohaveevolvedfromagroupA-likeMgeneby
6、atwostageprocessinvolvingabasesubstitutionintheintergenicregionwhichgeneratedanewAUGtranslationstartcodonfollowedbydeletionoftheoriginalAUG.IsolateUKllcloselyresembledMassstrainsintheintergenicregionbutwasdissimilarfromallstrainsintheproteincodingregion.TheMs
7、equencesofserotypesBandCwereidenticalandthoseoftheAandDserotypesverysimilar.Theseresultsarediscussedinrelationtorecentsequencingofpartofthespikeglycoproteingeneofsomeofthesestrainsandthediscoveryofinvitrorecombinationofmurinehepatitiscoronavirus.INTRODUCTIONI
8、nfectiousbronchitisvirus(IBV)isamajorcauseofdiseaseinthedomesticfowlandiseconomicallyveryimportanttothepoultryindustry.Intheyears1978to1983isolatesoffournewserotypes,definedonthebasisofne
