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1、VIROLOGY167,370-376(1988)SequenceAnalysisofthe3'EndoftheFelineCoronavirusFIPV79-1146Genome:ComparisonwiththeGenomeofPorcineCoronavirusTGEVRevealsLargeInsertionsRAOULJ.DEGROOT,1ARNOC.ANDEWEG,MARIANC.HORZINEK,ANDWILLYJ.M.SPAANInstituteofVirology,Veteri
2、naryFaculty,StateUniversityofUtrecht,Yalelaan1,3508TDUtrecht,TheNetherlandsReceivedMay5,1988;acceptedJuly29,1988Thegeneticinformation,carriedonmRNA6offelineinfectiousperitonitisvirus(FIPV)strain79-1146,wasdeter-minedbysequenceanalysisofcDNAclonesderi
3、vedfromthe3'endoftheFIPVgenome.TwoORFswerefound,encodingpolypeptidesof11K(ORF-1)and22K(ORF-2).TheFIPVsequencewascomparedtothe3'endsequenceoftransmissiblegastroenteritisvirus(TGEV).ORF-1hasahomologouscounterpart(ORF-X3)intheTGEVgenome;bothORFsarelocat
4、edatthesamepositionrelativetothenucleocapsidgene.However,asaresultofanin-frameinsertionordeletion,ORF-1is69nucleotideslargerthanORF-X3.AsimilareventhasoccurredimmediatelydownstreamofORF1:a624-nucleotidesegment,containingthecompleteORF-2,isabsentinthe
5、TGEVsequence.Mostsequencesimilarity(98.5%)wasfoundinthe3'noncodingsequences.ORF-X3andORF-1areprecededbythesequenceAAC-TAAAC,whichisassumedtobethetranscription-initiationsignalinFIPVandTGEV(P.A.KapkeandD.A.Brian(1986)Virology151,41-49).BySlnucleaseana
6、lysis,the5'endofFIPVRNA6wasmappedimmediatelyupstreamofthissequence.A700-nucleotideTGEV-specificRNAwasfoundbycross-hybridizationwithanFIPV3'endprobe,suggestingthatTGEVORF-X3isalsocarriedonaseparatemRNA.Thedifferencesatthe3'endsoftheFIPVandTGEVgenomesm
7、aybetheresultofRNArecombinationevents.©1988AcademicPress,Inc.INTRODUCTIONcatedasthecoronavirusesdiverged(deGrootetal.,1987a).Coronaviruses,agroupofenveloped,positive-Felineinfectiousperitonitisvirus(FIPV)andtransmis:strandedRNAviruses,haveattractedco
8、nsiderablein-siblegastroenteritisvirus(TGEV)ofswinebelongtotheterestbecauseoftheirunusualreplicationstrategy.Insameantigeniccluster(PedersenetaL,1978;Horzinektheinfectedcell,therearefivetosevensubgenomiceta/.,1982;SiddelletaL,1983)andarecloselyrelate