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1、首都医科大学学报JournalofCapitalMedicalUniversity•鼻生理功能/专题•转录因了Foxp3在小鼠变应性鼻炎外周表达的实验研究锡琳^韩德民匚2范尔钟匚2李颖1，2金善哲张伟1,2张罗1,2*(1•首都俟科大学附属北京同仁俟院耳鼻咽喉头颈外科；2•北京山•耳鼻咽喉科研究所，耳鼻咽喉头颈科学教育部重点实验室)【摘要】目的研究叉状头转录因子Foxp3在变应性鼻炎小鼠模型外周血单个核细peripheralbloodmonocyte,PBMC)中的表达并与正常小讯比较。方法选取8周雌性BALB/c小鼠30只，分为实验组和对照纽，
2、毎组15只。卵淸蛋白(ovalbumin,OVA)致敏激发，建立BALB/c小鼠变应性鼻炎模型：，21d后处理动物，取舜腔固定脱钙后染色,观察其形态学特点；取脾组织研磨后红细胞裂解液分离获得PBMC,提取RNA行逆转录•聚合酶链反应(reverseIranscriplion-polymerasechainreaction,RT-PCR)，检测Foxp3mRNA表达情况。Foxp3柑对表达量采用Foxp3与内参照GAPDH灰度比值表示。结果与对照纽相比，实验纽鼻腔黏膜部分脱落，黏膜下叫见浆液腺增生和嗜酸性粒细胞浸润。FoxP3/GAPDH灰度比，实
3、验组Foxp3niRNA表达明爪低于对照组，差异有统计学意义(P<0.05)°结论Foxp3mRNA在变应性疾病中表达下调,低于正常对照，提示Foxp3作为CD4+CD25+Treg细胞的特异性活化标志，在变应性疾病的发病机制中可能起重要调节作用。【关键词】变应性鼻炎；Foxp3；调节性T细胞；外周血单个核细胞【中图分类号】R765.21ExprimentalStudyonFoxp3mRNAExpressioninthePeripheralBloodMonocyteofMicewithAllergicRhinitisXiLin12,HanD(*m
4、in1,2,FanErzhong1,2,LiYing12,JinShanzhe12,ZhangWei12,ZhangLuo12*(1.Departmentof'OtolaryngologyHeadandNeckSurgery,BeijingTongrenHospital,CapitalMedicalUniversity^2.BeijinghislituleofOlolaryngologyKeyLaboratoryofOtolaryngologyHeadandNeckSurgery,MinistryojEducation)[ABSTRACT]Obj
5、ectiveToinvestigatetheleveloftranscriptionfactorFoxp3rhinitislevelinlheperipheialbloodmonocyte(PBMC)ofmicewithallergicrhinitis,andilsrelationshipwithpathogenofallergicAR.Methods30femaleBALB/cmiceweredividedinto2groups：ovalbumin()gioupandcontnd.Afler21daystheARmodelswereestabl
6、ished・Thepathologicaldifferencesl>etweenlhetwogroupswerethensurveyed・Reversetranscription-polymerasechainreaction(RT-PCR)wasusedtoexamineFoxp3mRNAexpressioninthespleenPBMCfromthetwogroups.ResultsIncontrasttothecontrol,OAmodelshadsignificantpathologicalchangesinlhenasalcavity
7、,includingepithelialdamageandeosinophilinfiltrationinlhesubmucosa・Foxp3mRNAexpressionwaslowerinsubjectswithARthanthatinthecontrols(P<0.05).ConclusionTheexpressionofFoxp3mRNAin(heOVAgroupislowwhichsuggeststhatFoxp3mayplayanimportantregulatoryroleinthepathogenesisofAR・[KEYWORDS
8、]allergicrhinitis；Foxp3；regulatoryTcells；peripheralbloodmonocyteCI)4
