资源描述：
《【精品】水稻遗传研究动态资料.docx》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、(五)水稻遗传研究动态(牵头与起草专家：庄杰云I専士等)1、近5年水稻遗传研究概述2、2007年我国水稻遗传研究动态研究3、2007年国际水稻遗传研究动态4、水稻遗传研究发展趋势001《PlantCellReports》Thericepathogen-relatedprotein10(JIOsPRIO)isinducedbyabioticandbioticstressesandexhibitsribonucleaseactivitySunlacKim,ScokYu,YoungHyunKang,SangGonKim,Jac・YcanKim,Sun-HyungKim,KyuYoungKa
2、ngEnvironmcntalBiotechnologyNationalCoreResearchCenter,GyeongsangNationalUniversity,Jinju,660-701,KoreaAbstract:Wepreviouslyreportedthatriceblastfungusorjasmonicacidinducedtheexpressionofricepathogenesis-relatedclass10(JIOsPRIO)proteins(Kimctal.2003,2004).However,nofurtherstudieshavebccncarried
3、outtoexaminetheexpression,localization,andenzymaticactivityofthisproteinineitherdevelopmentaltissuesorintissuesunderabioticstressconditions・Inthisstudy,riceJIOsPRIOwasexaminedbyWesternblotanalysis,immunolocalization,andbiochemicalassays.WesternblotsrevealedthattheJIOsPRIOproteinwasexpressedinde
4、velopmentaltissues,includinginflowerandroot.TIicproteinwasalsoexpressedunderabioticstresses,suchasoccursduringscncsccnccandwounding.Usingimmunohistochemicaltechniques,wedeterminedthatexpressionofJIOsPRIOwaslocalizedtothepaleaofflower,intheexodermis,andinnerpartoftheendodermisoftheroot.Insenesce
5、ncingtissuesofleafandcoleoptiles,itsexpressionwaslocalizedinvascularbundles・TheRNaseactivityusingJIOsPRIOrecombinantproteinwasdeterminedandabolishedaftertreatmentwithDTTinanativcin-gelassay.Totestthis,wccreatedJIOsPRIOmutantproteinscontainingserinesubstitutionsofaminoacidsC81S,C83S,orbothandexa
6、minedtheirRNaseactivities・TheactivityoftheC83Smutantwasdecreasedintheagarosegelassaycomparedtothewildtype・Takentogether,wehypothesizethattheJIOsPRIOproteinpossessesRNaseactivitythatissensitivetoDTEsuggestingtheimportanccoftlicdisulfidebondingbctwccncysteineresiduesandtliatitmightplayaroleincons
7、titutiveself-defensemechanismsinplantsagainstbioticandabioticstresses・002《Planta》ExpressionofriceheatstresstranscriptionfactorOsHsfA2eenhancestolerancetoenvironmentalstressesintransgenicArabidopsisNaokiY^kotani,TakanariIchikawa,Yo
