常用缓冲溶液的配制-(17698).pdf

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1、常用缓冲溶液的配制方法1．甘氨酸–盐酸缓冲液（0.05mol/L）X毫升0.2mol/L甘氨酸+Y毫升0.2mol/LHCI，再加水稀释至200毫升pHXYpHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75.07，0.2mol/L甘氨酸溶液含15.01克/升。2．邻苯二甲酸–盐酸缓冲液（0.05mol/L）X毫升0.2mol/L邻苯二甲酸氢钾+0.2mol/LHCl，再加水稀释到20毫升pH(20℃)XYpH(20℃)XY2.254.0703.251.4702.453.9603.4

2、50.9902.653.2953.650.5972.852.6423.850.2633.052.022邻苯二甲酸氢钾分子量=204.23，0.2mol/L邻苯二甲酸氢溶液含40.85克/升3．磷酸氢二钠–柠檬酸缓冲液pH0.2mol/L0.1mol/LpH0.2mol/L0.1mol/LNa2HPO4柠檬酸Na2HPO4柠檬酸（毫升）（毫升）（毫升）（毫升）2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.37

3、3.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55Na2HPO4分子量=14.98，0.2mol/L溶液为28.40克/升。Na2HPO4·2H2O分子量=178.05，

4、0.2mol/L溶液含35.01克/升。C4H2O7·H2O分子量=210.14，0.1mol/L溶液为21.01克/升。4．柠檬酸–氢氧化钠-盐酸缓冲液pH钠离子浓度柠檬酸（克）氢氧化钠(克)盐酸（毫升）最终体积①（mol/L）C6H8O7·H2ONaOH97%HCl（浓）（升）2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610①使用时可以每升中加入1克克酚，若最后pH值有变化，再用少量50%氢氧化钠溶液

5、或浓盐酸调节，冰箱保存。5．柠檬酸–柠檬酸钠缓冲液（0.1mol/L）pH0.1mol/L0.1mol/LpH0.1mol/L0.1mol/L柠檬酸柠檬酸钠柠檬酸柠檬酸钠（毫升）（毫升）（毫升）（毫升）3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8柠檬酸C6H8O7

6、·H2O：分子量210.14，0.1mol/L溶液为21.01克/升。柠檬酸钠Na3C6H5O7·2H2O：分子量294.12，0.1mol/L溶液为29.41克/毫升。6．乙酸–乙酸钠缓冲液（0.2mol/L）0.2mol/L0.3mol/L0.2mol/L0.3mol/LpH(18℃)NaAcHAcpH(18℃)NaAcHAc(毫升)(毫升)(毫升)(毫升)2.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905

7、.105.89.400.60Na2Ac·3H2O分子量=136.09，0.2mol/L溶液为27.22克/升。7．磷酸盐缓冲液（1）磷酸氢二钠–磷酸二氢钠缓冲液（0.2）0.2mol/L0.3mol/L0.2mol/L0.3mol/LpHNa2HPO4NaH2PO4pHNa2HPO4NaH2PO4(毫升)(毫升)(毫升)(毫升)5.88.092.07.061.039.05.910.090.07.1