常用缓冲溶液的配制方法.pdf

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1、常用缓冲溶液的配制方法1.磷酸氢二钠–柠檬酸缓冲液··········································································22．柠檬酸–柠檬酸钠缓冲液（0.1mol/L）··························································23．甘氨酸–盐酸缓冲液（0.05mol/L）·······························································34．邻苯二甲酸–盐酸缓冲液（0.05

2、mol/L）·························································35．乙酸–乙酸钠缓冲液（0.2mol/L）································································36．磷酸盐缓冲液·························································································37．磷酸二氢钾–氢氧化钠缓冲液（0.05M）················

3、········································48．巴比妥钠-盐酸缓冲液（18℃）····································································49．硼酸–硼砂缓冲液（0.2M硼酸根）·······························································510．甘氨酸–氢氧化钠缓冲液（0.05M）·······················································

4、······511．碳酸钠-碳酸氢钠缓冲液（0.1M）·······························································512．Tris–盐酸缓冲液（0.05M，25℃）······························································613．硼砂-氢氧化钠缓冲液（0.05M硼酸根）·······················································614．PBS缓冲液····················

5、·······································································615.B-R缓冲溶液配制···················································································716.常用pH缓冲溶液的配制和pH值································································711.磷酸氢二钠–柠檬酸缓冲液0.2mol/L0.1mol/L柠檬酸0

6、.2mol/L0.1mol/L柠檬酸pHpHNa2HPO4(mL)(mL)Na2HPO4(mL)(mL)2.20.4019.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.

7、473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55Na2HPO4分子量=14.98，0.2mol/L溶液为28.40克/升。Na2HPO4·2H2O分子量=178.05，0.2mol/L溶液含35.01克/升。C4H2O7·H2O分子量=210.14，0.1mol/L溶液为21.01克/升。2．柠檬酸–柠檬酸钠缓冲液（0.1mol/L）0.1mol/L0.1mol