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时间:2018-01-12
《常用缓冲溶液的配制方法》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、常用缓冲溶液的配制方法1.甘氨酸–盐酸缓冲液(0.05mol/L)X毫升0.2mol/L甘氨酸+Y毫升0.2mol/LHCI,再加水稀释至200毫升pHXYpHXY2.02.42.62.85050505044.032.424.216.83.03.23.43.65050505011.48.26.45.0甘氨酸分子量=75.07,0.2mol/L甘氨酸溶液含15.01克/升。 2.邻苯二甲酸–盐酸缓冲液(0.05mol/L)X毫升0.2mol/L邻苯二甲酸氢钾+0.2mol/LHCl,再加水稀释到20毫升pH(20℃)XYp
2、H(20℃)XY2.22.42.62.83.0555554.0703.9603.2952.6422.0223.23.43.63.855551.4700.9900.5970.263邻苯二甲酸氢钾分子量=204.23,0.2mol/L邻苯二甲酸氢溶液含40.85克/升 3.磷酸氢二钠–柠檬酸缓冲液pH0.2mol/LNa2HPO4(毫升)0.1mol/L柠檬酸(毫升)pH0.2mol/LNa2HPO4(毫升)0.1mol/L柠檬酸(毫升)2.22.42.62.83.03.23.43.63.84.04.24.44.64.85.
3、00.401.242.183.174.114.945.706.447.107.718.288.829.359.8610.3010.6018.7617.8216.8315.8915.0614.3013.5612.9012.2911.7211.1810.6510.149.705.25.45.65.86.06.26.46.66.87.07.27.47.67.88.010.7211.1511.6012.0912.6313.2213.8514.5515.4516.4717.3918.1718.7319.1519.459.288.8
4、58.407.917.376.786.155.454.553.532.611.831.270.850.55Na2HPO4分子量=14.98,0.2mol/L溶液为28.40克/升。Na2HPO4·2H2O分子量=178.05,0.2mol/L溶液含35.01克/升。C4H2O7·H2O分子量=210.14,0.1mol/L溶液为21.01克/升。 4.柠檬酸–氢氧化钠-盐酸缓冲液pH钠离子浓度(mol/L)柠檬酸(克)C6H8O7·H2O氢氧化钠(克)NaOH97%盐酸(毫升)HCl(浓)最终体积(升)①2.23.13.
5、34.35.35.86.50.200.200.200.200.350.450.3821021021021024528526684838383144186156160116106456810512610101010101010① 使用时可以每升中加入1克克酚,若最后pH值有变化,再用少量50% 氢氧化钠溶液或浓盐酸调节,冰箱保存。5.柠檬酸–柠檬酸钠缓冲液(0.1mol/L)pH0.1 mol/L柠檬酸(毫升)0.1 mol/L柠檬酸钠(毫升)pH0.1 mol/L柠檬酸(毫升)0.1 mol/L柠檬酸钠(毫
6、升)3.03.23.43.63.84.04.24.44.64.818.617.216.014.914.013.112.311.410.39.21.42.84.05.16.06.97.78.69.710.85.05.25.45.65.86.06.26.46.68.27.36.45.54.73.82.82.01.411.812.713.614.515.316.217.218.018.6柠檬酸C6H8O7·H2O:分子量210.14,0.1mol/L溶液为21.01克/升。柠檬酸钠Na3C6H5O7·2H2O:分子量294.1
7、2,0.1mol/L溶液为29.41克/毫升。 6.乙酸–乙酸钠缓冲液(0.2mol/L) pH(18℃)0.2 mol/LNaAc(毫升)0.3 mol/LHAc(毫升) pH(18℃)0.2 mol/LNaAc(毫升)0.3 mol/LHAc(毫升)2.63.84.04.24.44.60.751.201.802.653.704.909.258.808.207.356.305.104.85.05.25.45.65.85.907.007.908.609.109.404.103.002.101.400.900.60
8、Na2Ac·3H2O分子量=136.09,0.2mol/L溶液为27.22克/升。7.磷酸盐缓冲液(1)磷酸氢二钠–磷酸二氢钠缓冲液(0.2) pH0.2 mol/LNa2HPO4(毫升)0.3 mol/LNaH2PO4(毫升) pH0.2 mol/LNa2HPO4(毫升)0.3 mol/LNaH2PO4(毫
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