常用缓冲溶液的配制

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1、常用缓冲溶液的配制（二）邻苯二甲酸-盐酸缓冲液（0.05mol/L）X毫升0.2mol/L邻苯二甲酸氢钾＋Y毫升0.2mol/LHCl，再加水稀释至20毫升。pH(20℃)XYpH(20℃)XY2.254.6703.251.4702.453.9603.450.9902.653.2952.650.5972.852.6423.850.2633.052.032邻苯二甲酸氢钾分子量＝204.23。0.2mol/L邻苯二甲酸氢钾溶液含40.85g/L。（六）醋酸-醋酸钠缓冲液（0.2mol/L）pH(18℃)0.2mol/LNaAc(mL)0.2mol/LHAc(mL)pH(18℃

2、)0.2mol/LNaAc(mL)0.2mol/LHAc(mL)3.60.759.354.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.86.400.60NaAc·3H2O分子量＝136.09；0.2mol/L溶液为27.22g/L。冰乙酸11.8mL稀释至1L（需标定）。（七）磷酸二氢钾-氢氧化钠缓冲液（0.05mol/L）X毫升0.2mol/LKH2PO4+Y毫升0.2mol/LNaOH加水稀释至

3、20毫升。pH(20℃)X(mL)Y(mL)pH(20℃)X(mL)Y(mL)5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.680（八）磷酸盐缓冲液磷酸氢二钠-磷酸二氢钠缓冲液（0.2mol/L）pH0.2mol/LNa2HPO4(mL)0.2mol/LNaH2PO4(mL)pH0.2mol/LNa2HPO4(mL)0.2mol/LNaH2PO4(mL)5.88.092.07.061.039.05.910.09

4、0.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.051.08.094.75.36.955.045.0Na2HPO4·2H2O分子量＝178.05；0.2mol/L溶液为35.61g/L。Na2HPO4·12H2O分子量＝358.22；0.

5、2mol/L溶液为71.64g/L。NaH2PO4·H2O分子量＝138.01；0.2mol/L溶液为27.6g/L。NaH2PO4·2H2O分子量＝156.03；0.2mol/L溶液为31.21g/L。（十一）、硼酸-硼砂缓冲液(0.2mol/L硼酸根)pH0.05mol/L硼砂(mL)0.2mol/L硼酸(mL)pH0.05mol/L硼砂(mL)0.2mol/L硼酸(mL)7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂：Na2B4O7·10H2O分子量＝381.43；

6、0.05mol/L溶液（等于0.2mol/L硼酸根）含19.07g/L。硼酸：H3BO3分子量＝61.84；0.2mol/L的溶液为12.37g/L。硼砂易失去结晶水，必须在带塞的瓶中保存。（十三）硼砂-氢氧化纳缓冲液（0.05mol/L硼酸根）X毫升0.05mol/L硼砂＋Y毫升0.2mol/LNaOH加水稀释至200毫升。pHX(mL)Y(mL)pHX(mL)Y(mL)9.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂Na2B4O7·10H2O分子量＝381.43；0.05mol/L硼砂溶液（等于0.2m

7、ol/L硼酸根）为19.07g/L。（十四）碳酸钠-碳酸氢钠缓冲液（0.1mol/L）(此缓冲液在Ca2＋、Mg2＋存在时不得使用).pH0.1mol/LNa2CO3(mL)0.1mol/LNaHCO3(mL)20℃37℃9.168.77199.409.22289.519.40379.789.50469.909.725510.149.906410.2810.087310.5310.288210.8310.5791Na2CO3·10H2O分子量＝286.2；0.1mol/L溶液为28.62g/L。NaHCO3分子量＝84.0；0