GM9328P-1989抗霉性-混合培养方法.pdf

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1、VND.GMGM932AP-ENGL1989m900b3790032774Cl09-GENERALMOTORSENGINEERINGSTANDAMaterialsandProcesses-ProceduresMILDEWRESISTANCE-MIXEDCULTUREMETHODGH9328P1SCOPE.T’hismethodisintendedforevaluatinganti-refrigeratorsat3to7Candtransfersmadeat6monthfungalcffcctivmusofplastics,coatedfabricsandotherinter

2、vals.Subculturesincubatedat28Cfor7to14dayspoiymericmateriakshouldbeusedinpreparingthesporesuspension.2TESTEQUIPMENTANDMATERIALS.4.2MixaiFungalSporeSuspension.2.1Disposablepetridishes.100x100x25mmarerccom-4.2.1Preparation.Asporesuspensionispreparedbypour-mcrdcd,anyconvenientsiremaybeused.Av

3、ailablefrom:ingintoonesubcultureofeachorganisma10mLofsterileTri-StatePlasticMoldingCo.,CatalogX91,Hendersondistilledwatercontaining0.05mL/LofnontoxicwettingKY42420.agentsuchassodiumdicxtylsulfosuccinate.Useasterileplatinumornichromeinoculatinglooptogentlyscrapethe2.2Autoclavecapableofmaint

4、ainingasteampressureofsurfacegrowthfromtheagarmedium.100kPaatanexhausttanperatureof121C.4.22Pourthesporemixturesintoasterilejarcontaining102.3Incubatorcapableofmaintainingatemperatureofto15solidglassbeads,5mmindiameter,andshake2821C.vigorouslytobreakupthesporelumps.2.4Atomizer:DeViIbii+15,

5、ora@ivaknt.4.2.2.1Filtertheshakensuspensionthroughathinlayerofsterileglasswoolinastcriiefunnelintoasterilebottle.Add3CULTUREMEDIA.distilledwatertomakeupthevolumeof1OomL.3.1Thetestmediumcompositionshallbeasfollows.Donot4.22.2ThesporesuspensionmaybepreparedfresheachadjustthepH.dayormaybekept

6、inarcfiigcratorat3to10Cfornotmorethan7days.AmmoniumnitratemNO3........................3.0gPotassiumdihyclrogcnorthophosphatc@&PO,)..,2.0g5PROCEDURE.Magnesiumsulfate(MgSO,7HzO)....................0.5gPotassiumchloride(-KCl)..............................0.5g5.1TestSpecimen.Cuta25mmdiameterdi

7、scfromeachDextrose...................................-....-.........1.0gsampleandplaceonthetestmedium.Forcoatedfabrics,2Agar..................._.................................15.Ogdiscscutfromeachsamplearetested.withtheplasticsideDistilledwafer..............