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1、猪传染性胸膜肺炎放线杆菌的分离鉴定、致病性与药敏试验摘要:从湖北省几个猪场具有严重呼吸道症状的疑似病例中,采集了肺脏、扁桃体、关节液及心包液等病料,进行了细菌的分离培养及菌落形态观察、染色镜检、生化试验、PCR检验、血清型鉴定、药敏试验及致病性试验。结果表明,分离到的8株细菌为猪传染性胸膜肺炎放线杆菌(Actinobac订luspleuropneumoniae,APP)。血清型分型鉴定结果为1型的6株,3型的2株。药敏试验结果显示,分离的菌株对头胞类、先锋霉素类表现较高的敏感性,为猪场用药提供了科学参考。关键词:猪传染性胸膜肺炎放线杆菌(Actinoba
2、c订luspleuropneumoniae,APP);鉴定;耐药性;致病性中图分类号:S858.28文献标识码:A文章编号:0439-8114(2014)21-5204-04D0I:10.14088/j.cnki.issn0439-8114.2014.21.040IsolationIdentificationandMedicineSensitivityofActinobcillusp1europneumoniaeTIANYong-xiangl,TANYong-qiang2,LIUZe~wenl,GUORuil,DUANZheng-yingl,YANGKe
3、-lil,ZHOUDan-nal,LUOYong-mei3,YUANFang-yanl(1.InstituteofAnimalHusbandryandVeterinary,HubeiAcademyofAgriculturalSciences/HubeiKeyLaboratoryofAnimalEmbryoandMolecularBreeding,Wuhan430064,China;2.CollegeofVeterinaryMedicine,HuazhongAgriculturalUniversity,Wuhan430070,China;3.Xiangya
4、ngAnimalHea1thSupervisionStation,Xiangyang442110,Hubei,China)Abstract:Thesamplesoflungs,tonsils,jointfluidsandpericardialfluidswerecollectedfromsomesuspectedswinewithserioussymptomsofrespiratoryinHubeiprovinceandconductedcultureandidentificationthroughcolonialmorphology,observati
5、on,stainingmicroscopy,biochemistryqualification,PCR,serotyping,drugsensitivityandpathogenicityexamination.Theresultsshowedthatthe8isolatedstrainsofbacteriawereidentifiedasporcinecontagiousActinobacilluspleuropneumoniae・Theserotypewas6ofserotype1and2ofserotype3.Resultsofdrugsuscep
6、tibilitytestshowedthattheisolatedstrainshadhighsensitivitywithcephalosporinsandcephalothiri.Itwillprovideascientificreferenceforfarmmedication.Keywords:Actinobac订luspleuropneumoniae;identification.;antimicrobialresistance;pathogenicity猪传染性胸膜肺炎(Porcinecontagiouspleuropneumia,PCP)是
7、由胸膜肺炎放线杆菌(Actinobac订luspleuropneumoniae,APP)引起的一种高度传染性呼吸道疾病,能引起猪的胸膜肺炎和肺炎等。随着养猪业的规模化、集约化发展,该病已经成为严重危害养猪业发展的主要呼吸道疾病之一[1]。根据APP生长是否需要烟酰胺腺p票吟二核昔酸(NicotinamideAdeninedinucleotide,NAD),分为两个生物型,即生物I型(NAD依赖菌株)和生物II型(非NAD依赖型),根据APP表面荚膜多糖和脂多糖抗原性的不同,又分为15个血清型,生物I型包括血清型1T2型和15型[2]。本研究对湖北省武汉市
8、、宜昌市等地的多个猪场采集了疑似病猪的肺脏、关节液等病料,进行了细菌分离培养及鉴